Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1355)
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Table of contents (23 protocols)
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Front Matter
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Techniques for Tagging Phospho-Moieties
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Front Matter
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Labeling Techniques for Quantitative Phosphoproteomics
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Front Matter
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Phosphopeptide Enrichment Strategies
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Front Matter
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Phosphoprotein Analysis by Mass Spectrometry
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Front Matter
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About this book
This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
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University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark
Louise von Stechow
Bibliographic Information
Book Title: Phospho-Proteomics
Book Subtitle: Methods and Protocols
Editors: Louise von Stechow
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3049-4
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3048-7Published: 20 November 2015
Softcover ISBN: 978-1-4939-5001-0Published: 23 August 2016
eBook ISBN: 978-1-4939-3049-4Published: 19 November 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIII, 364
Topics: Protein Science, Proteomics