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Assessment of Mitochondrial Ca2+ Uptake

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1264))

Abstract

Mitochondrial Ca2+ uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca2+ signals and elaborating the mechanisms that accomplish mitochondrial Ca2+ uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca2+ concentration ([Ca2+]mito) in a wide range of applications.

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Authors and Affiliations

  1. Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010, Graz, Austria

    András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier & Markus Waldeck-Weiermair

Authors
  1. András T. Deak
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  2. Claire Jean-Quartier
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  3. Alexander I. Bondarenko
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  4. Lukas N. Groschner
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  5. Roland Malli
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  6. Wolfgang F. Graier
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  7. Markus Waldeck-Weiermair
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Corresponding author

Correspondence to Markus Waldeck-Weiermair .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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Deak, A.T. et al. (2015). Assessment of Mitochondrial Ca2+ Uptake. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_35

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  • DOI: https://doi.org/10.1007/978-1-4939-2257-4_35

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2256-7

  • Online ISBN: 978-1-4939-2257-4

  • eBook Packages: Springer Protocols

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