Abstract
Cryopreservation is a valuable technique for aquaculture as it enables a library or bank of genetically valuable animals to be maintained in a cost-effective manner. Here, we describe a method to cryopreserve the sperm of the Greenshell™ mussel (Perna canaliculus) and how to use the sperm post-thawing to maximize larval production from thawed sperm in selective breeding.
Key words
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
McFadzen IRB (1995) Cryopreservation of sperm of the Pacific oyster Crassostrea gigas. Methods Mol Bio 38:145–149
Adams SL, Smith JF, Roberts RD, Jankec AR, Kaspar HF, Tervit HR, Pugh PA, Webb SC, King NG (2004) Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture 242:271–282
Tervit HR, Adams SL, Roberts RD, McGowan LT, Pugh PA, Smith JF, Janke AR (2005) Successful cryopreservation of Pacific oyster (Crassostrea gigas) oocytes. Cryobiology 51:142–151
Tiersch TR, Yang H, Jenkins JA, Dong Q (2007) Sperm cryopreservation in fish and shellfish. Soc Reprod Fertil Suppl 65:493–508
Adams SL, Smith JF, Roberts RD, Janke AR, King NG, Tervit HR, Webb SC (2008) Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg). Aquacult Res 39:1434–1442
Environment Agency UK (2007) The direct toxicity assessment of aqueous environmental samples using the oyster (Crassostrea gigas) embryo-larval development test: methods for the examination of waters and associated materials, Environment Agency UK, Bristol, UK. http://www.environment-agency.gov.uk/static/documents/Research/oyster209jan30_1388168.pdf. Accessed 26 Feb 2014.
Environment Canada (2011) Biological test method: fertilization assay using echinoids (Sea Urchins and Sand Dollars) EPS 1/RM/27, 2nd ed, Ottawa, Canada. http://www.ec.gc.ca/Publications/047B08AB-530E-49EA-8EC8-8AB8E8D75DA4/1_BiologicalTestMethodFertilizationAsayUsingEchinoidsSeaUrchinsSandDollars2ndEdition.pdf. Accessed 26 Feb 2014.
ASTM (2012) E724: standard guide for conducting static acute toxicity tests starting with embryos of four species of saltwater bivalve molluscs. ASTM International, West Conshohocken, PA
ASTM (2012) E1563: standard guide for conducting static acute toxicity tests with echinoid embryos. ASTM International, West Conshohocken, PA
Paredes E, Bellas J (2009) Cryopreservation of sea urchin embryos (Paracentrotus lividus) applied to marine ecotoxicological studies. Cryobiology 59:344–350
Paniagua-Chavez CG, Tiersch TR (2001) Laboratory studies of cryopreservation of sperm and trochophore larvae of the eastern oyster. Cryobiology 43:211–223
Adams SL, Hessian PA, Mladenov PV (2004) Cryopreservation of sea urchin (Evechinus chloroticus) sperm. Cryo Letters 25:287–299
Dong Q, Eudeline B, Huang C, Allen SK Jr, Tiersch TR (2005) Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oysters, Crassostrea gigas. Cryobiology 50:1–16
Adams SL, Smith JF, Tervit HR, McGowan LT, Roberts RD, Janke AR, King NG, Gale SL, Webb SC (2011) Cryopreservation of molluscan sperm: Pacific oyster, Green-lipped mussel and Paua abalone. In: Tiersch TR, Green CC (eds) Cryopreservation in aquatic species, 2nd edn. World Aquaculture Society, Baton Rouge, LA, pp 562–573
Smith JF, Adams SL, Gale SL, McGowan LT, Tervita HR, Roberts RD (2012) Cryopreservation of Greenshell™ mussel (Perna canaliculus) sperm. I. Establishment of freezing protocol. Aquaculture 334–337:199–204
Paredes E, Adams SL, Tervit HR, Smith JF, McGowan LT, Gale SL, Morrish JR, Watts E (2012) Cryopreservation of Greenshell™ mussel (Perna canaliculus) trochophore larvae. Cryobiology 65:256–262
Paredes E, Bellas J, Adams SL (2013) Comparative cryopreservation study on two species of bivalves: Pacific oyster (Crassostrea gigas) and blue mussel (Mytilus galloprovincialis). Cryobiology 67:274–279
Liu Y, Xu T, Robinson N, Qin J, Li X (2014) Cryopreservation of sperm in farmed Australian greenlip abalone Haliotis laevigata. Cryobiology 68:185–193
Aquaculture New Zealand (2012) New Zealand aquaculture: a sector overview with key facts, statistics and trends. http://aquaculture.org.nz/wp-content/uploads/2012/05/NZ-Aquaculture-Facts-2012.pdf. Accessed 13 Dec 2013.
Alfaro AC, Jeffs AG, Hooker SH (2001) Reproductive behaviour of the green-lipped mussel, Perna canaliculus, in northern New Zealand. Bull Mar Sci 69:1095–1108
Martinez-Pastor F, Adams SL (2008) Cryobiological material and handling procedures. In: Cabrita E, Robels V, Herraez P (eds) Methods in reproductive aquaculture: marine and freshwater species. CRC, Boca Raton, FL, pp 295–319
Adams SL, Tervit HR, McGowan LT, Smith JF, Roberts RD, Salinas-Flores L, Gale SL, Webb SC, Mullen SF, Critser JK (2009) Towards cryopreservation of Greenshell mussel (Perna canaliculus) oocytes. Cryobiology 58:69–74
Acknowledgment
This research was funded by the New Zealand Ministry of Business, Innovation, and Employment (CAWX0802 and CAWX1315). We thank SPATnz and Kono for supplying mussels and technical assistance and also thank the staff of Cawthron Aquaculture Park (in particular Jonathan Morrish and Ellie Watts) for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Adams, S.L., Smith, J.F., Taylor, J., McGowan, L.T., Tervit, H.R. (2015). Cryopreservation of Greenshell™ Mussel (Perna canaliculus) Sperm. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2193-5_14
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-2192-8
Online ISBN: 978-1-4939-2193-5
eBook Packages: Springer Protocols