Abstract
In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience’s competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm’s dynamic array high-throughput platform.
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Acknowledgments
This research was funded by the McKnight Foundation and Ezra Taft Benson Agriculture and Food Institute. We gratefully acknowledge Dr. Joshua Udall (BYU), Jared Clouse (BYU) and Jamie Rice (Fluidigm) for his assistance and advice with regards to the Fluidigm protocols.
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Smith, S.M., Maughan, P.J. (2015). SNP Genotyping Using KASPar Assays. In: Batley, J. (eds) Plant Genotyping. Methods in Molecular Biology, vol 1245. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1966-6_18
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DOI: https://doi.org/10.1007/978-1-4939-1966-6_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1965-9
Online ISBN: 978-1-4939-1966-6
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