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Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

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Book cover Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1194))

Abstract

During the development of the hematopoietic system, at least eight distinct lineages are generated in the mouse embryo. Transgenic mice expressing fluorescent proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to multipotent progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene and survey available fluorescent probes and methods for confirming and analyzing transgene expression in the hematopoietic systems of the embryo, fetus, and postnatal/adult animal.

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Acknowledgements

We thank J. Barminko and G. Camprecios for their comments on the manuscript. Work in our laboratory was supported in part by grants to M.H.B. from the National Institutes of Health (RO1 HL62248 and, DK52191, and EB02209), the Roche Foundation for Anemia Research (grant 9699367999, cycle X), and the New York State Department of Health (NYSTEM grant N08G-024).

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Authors and Affiliations

  1. Department of Medicine, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, 1079, New York, NY, 10029-6574, USA

    Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves & Margaret H. Baron M.D., Ph.D.

  2. Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA

    Margaret H. Baron M.D., Ph.D.

  3. Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA

    Margaret H. Baron M.D., Ph.D.

  4. The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA

    Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves & Margaret H. Baron M.D., Ph.D.

  5. The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA

    Margaret H. Baron M.D., Ph.D.

  6. Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA

    Margaret H. Baron M.D., Ph.D.

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  1. Andrei M. Vacaru
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  2. Joseph Vitale
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  3. Johnathan Nieves
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  4. Margaret H. Baron M.D., Ph.D.
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Corresponding author

Correspondence to Margaret H. Baron M.D., Ph.D. .

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Editors and Affiliations

  1. Stem Cell Regulation and Animal Aging Section, Basic Research Laboratory,, National Cancer Institute, National Institutes of Health, Frederick, MD, USA

    Shree Ram Singh

  2. Genetically Engineered Mouse Modeling Core/Department of Molecular Virology, Immunology and Medical Genetics, Wexner Medical Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Vincenzo Coppola

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Vacaru, A.M., Vitale, J., Nieves, J., Baron, M.H. (2014). Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development. In: Singh, S., Coppola, V. (eds) Mouse Genetics. Methods in Molecular Biology, vol 1194. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1215-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1215-5_16

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