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Quantitative Metabolomics Using ID-MS

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Metabolic Flux Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1191))

Abstract

Quantitative intracellular metabolite measurements are essential for systems biology and modeling of cellular metabolism. The MS-based quantification is error prone because (1) several sampling processing steps have to be performed, (2) the sample contains a complex mixture of partly compounds with the same mass and similar retention time, and (3) especially salts influence the ionization efficiency. Therefore internal standards are required, best for each measured compound. The use of labeled biomass, 13C extract, is a valuable tool, reducing the standard deviations of intracellular concentration measurements significantly (especially regarding technical reproducibility). Using different platforms, i.e., LC-MS and GC-MS, a large number of different metabolites can be quantified (currently about 110).

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands

    S. Aljoscha Wahl, Reza Maleki Seifar, Angela ten Pierick, Cor Ras, Jan C. van Dam, Joseph J. Heijnen & Walter M. van Gulik

Authors
  1. S. Aljoscha Wahl
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  2. Reza Maleki Seifar
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  3. Angela ten Pierick
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  4. Cor Ras
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  5. Jan C. van Dam
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  6. Joseph J. Heijnen
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  7. Walter M. van Gulik
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Corresponding author

Correspondence to S. Aljoscha Wahl .

Editor information

Editors and Affiliations

  1. Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management Centre, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Jens O. Krömer

  2. AIBN, University of Queensland, St. Lucia, Brisbane, Queensland, Australia

    Lars K. Nielsen

  3. Biology Department, RWTH Aachen University, Aachen, Germany

    Lars M. Blank

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© 2014 Springer Science+Business Media New York

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Wahl, S.A. et al. (2014). Quantitative Metabolomics Using ID-MS. In: Krömer, J., Nielsen, L., Blank, L. (eds) Metabolic Flux Analysis. Methods in Molecular Biology, vol 1191. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1170-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1170-7_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1169-1

  • Online ISBN: 978-1-4939-1170-7

  • eBook Packages: Springer Protocols

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