Abstract
Polymerase chain reaction (PCR) is an invaluable diagnostic technique in microbiology for rapid and specific detection and confirmation of microbial isolates from food and the environment. PCR is a simple, sensitive, specific, and reproducible assay and can be performed in conventional or in real-time formats. Here, we describe the application of real-time and conventional PCR-based methods for confirmation of presumptive Listeria isolates.
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References
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Rodríguez-Lázaro, D., Hernández, M. (2014). Confirmation of Isolates of Listeria by Conventional and Real-Time PCR. In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_3
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DOI: https://doi.org/10.1007/978-1-4939-0703-8_3
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