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Immunological Studies on Lysosomal Sphingomyelinase: Immunization Procedures, Properties of Polyclonal and Monoclonal Antibodies Obtained and Effect of Triton X-100 on Binding of Enzyme Activity

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Enzymes of Lipid Metabolism II

Part of the book series: NATO ASI Series ((NSSA,volume 116))

Abstract

Sphingomyelinase hydrolyses sphingomyelin to ceramide and phosphoryl choline. In tissues from patients suffering from Niemann-Pick disease type A and B this lysosomal, membrane-associated enzyme is deficient and sphingomyelin accumulates1. In tissues from patients with Niemann-Pick disease type C, sphingomyelin also accumulates but sphingomyelinase is not deficient1.

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References

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Author information

Authors and Affiliations

  1. Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD, Amsterdam, The Netherlands

    E. J. M. Al, J. M. Tager & A. W. Schram

  2. Section for Tumor Biology, Netherlands Cancer Institute, Amsterdam, The Netherlands

    E. J. M. Al & J. H. M. Hilgers

  3. Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagkstrasse 1, D-5300, Bonn, Federal Republic of Germany

    G. Weitz & K. Sandhoff

  4. Clincal Investigations and Therapeutics Section and Molecular and Medical Genetics Section, Developmental and Metabolic Neurology Branch, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20205, USA

    J. A. Barranger

Authors
  1. E. J. M. Al
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  2. G. Weitz
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  3. K. Sandhoff
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  4. J. A. Barranger
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  5. J. H. M. Hilgers
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  6. J. M. Tager
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  7. A. W. Schram
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Editor information

Editors and Affiliations

  1. CNRS Center for Neurochemistry and INSERM U44, Louis Pasteur University, Strasbourg, France

    Louis Freysz , Henri Dreyfus  & Raphaël Massarelli ,  & 

  2. Hebrew University-Hadassah School of Medicine, Jerusalem, Israel

    Shimon Gatt

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© 1986 Plenum Press, New York

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Al, E.J.M. et al. (1986). Immunological Studies on Lysosomal Sphingomyelinase: Immunization Procedures, Properties of Polyclonal and Monoclonal Antibodies Obtained and Effect of Triton X-100 on Binding of Enzyme Activity. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_39

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  • DOI: https://doi.org/10.1007/978-1-4684-5212-9_39

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5214-3

  • Online ISBN: 978-1-4684-5212-9

  • eBook Packages: Springer Book Archive

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