Skip to main content
SpringerLink
Log in

The Hordeins of Barley: Developmentally and Nutritionally Regulated Multigene Families of Storage Proteins

  • Chapter
Structure and Function of Plant Genomes

Part of the book series: NATO Advanced Science Institutes Series ((NSSA,volume 63))

Abstract

The endosperm of the grain plays an important role in the life cycle of a cereal plant. It develops over a period of some 40–50 days, finally containing about 70% of the total dry matter and nitrogen of the plant. About 50% of the N in the barley endosperm is in the form of prolamin storage proteins (termed hordeins). At the end of development the grain dries out and the bulk of the endosperm tissue (all except the outer aleurone layer which does not contain hordeins) dies. The endosperm therefore provides an example of a highly specialised tissue which has differentiated to carry out an important and unique function over a limited period of time (see1 for a further review). The hordeins have been studied because of their influence on nutritional quality2 and because they are the products of tissue-specific genes. This report summarizes our studies on the nature of the proteins, the genes specifying them and factors controlling the expression of these genes. We have pursued this work using the classical techniques of protein chemistry3,4,5 and genetics6,7 and also by constructing a cDNA library made from poly A+ RNA isolated from developing endosperms.8

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. B. M. Johri, P. S. Srivastava, and A. P. Raste, Endosperm Culture, in “International Review of Cytology, Supplement 11B,” G. H. Bourne, J. F. Danielli, eds., Academic Press, New York (1980).

    Google Scholar 

  2. P. R. Shewry, B. J. Miflin, B. G. Forde, S. W. J. Bright Conventional and novel approaches to the improvement of the nutritional quality of cereal and legume seeds, Sei. Prog. 67: 571 (1981).

    Google Scholar 

  3. A. J. Faulks, P. R. Shewry and B. J. Miflin, The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor 2 locus in barley (Hordeum vulgäre L.), Biochem. Genet. 19:841 (1981).

    Article  PubMed  CAS  Google Scholar 

  4. P. R. Shewry, E. J-L. Lew, D. D. Kasarda, Structural homology of storage proteins coded by the Hor 1 locus of barley (Hordeum vulgäre L.), Planta 153:246 (1981).

    Article  CAS  Google Scholar 

  5. P. R. Shewry and B. J. Miflin, Characterization and synthesis of barley seed proteins, in “The Genetics of Seed Proteins of Cereals and Legumes”. W. Gottschalk, H. P. Muller, eds. Martinus Nijhoff, The Hague (in press).

    Google Scholar 

  6. P. R. Shewry, A. J. Faulks, R. A. Pickering, I. T. Jones, R. A. Finch and B. J. Miflin The genetic analysis of barley storage proteins, Heredity 44: 383 (1980).

    Article  CAS  Google Scholar 

  7. P. R. Shewry and B. J. Miflin, Genes for the storage proteins of barley. Qual. Plant. (PI. Foods for Human Nutrition), (in press).

    Google Scholar 

  8. B. G. Forde, M. Kreis, M. B. Bahramian, J. Matthews, B. J. Miflin, R. D. Thompson, R. D. Bartels and R. B. Flavell Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein-related clones, Nucleic Acids Research 9: 6689 (1981).

    Article  PubMed  CAS  Google Scholar 

  9. B. J. Miflin, J. M. Field, and P. R. Shewry, Cereal storage proteins and their effects on technological properties in “Seed Proteins” J. Daussant, J. Mossé eds., Academic Press, London (in press).

    Google Scholar 

  10. J. M. Field, P. R. Shewry, B. J. Miflin and J. P. March The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley, Theor. Appl. Genet. 62: 329 (1982).

    CAS  Google Scholar 

  11. G. Salcedo, R. Sanchez-Monge, A. Aragamentaria and C. Aragoncillo The A. hordeins as a group of salt-soluble hydrophobic proteins, Plant Sei. Lett. 19: 109 (1980).

    Article  CAS  Google Scholar 

  12. P. R. Shewry, H. M. Pratt, A. J. Faulks, S. Parmar, and B. J. Miflin The storage protein (hordein) pattern of barley in relation to varietal identification and disease resistance, J. natn. Inst. agric. Bot. 15: 34 (1979).

    Google Scholar 

  13. J. Jensen, J. H. Jorgensen, H. P. Jensen, H. Giese, and H. Doll Linkage of the hordein loci Hor 1 and Hor 2 with the powdery mildew resistance loci Mlk and Mla on barley chromosome 5, Theor. Appl. Genet. 58: 27 (1980).

    CAS  Google Scholar 

  14. G. J. Lawrence and K. W. Shepherd Chromosomal location of genes controlling seed proteins in species related to wheat, Theor. Appl. Genet. 59: 25 (1981).

    CAS  Google Scholar 

  15. R. P. Ricciardi, J. S. Miller and B. E. Roberts Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation, Proc. Natl. Acad. Sei. USA 76: 4927 (1979).

    Article  CAS  Google Scholar 

  16. S. Rahman, P. R. Shewry and B. J. Miflin Differential protein accumulation during barley grain development. J. exp. Bot. 33: 717 (1982).

    Article  CAS  Google Scholar 

  17. P. R. Shewry, H. M. Pratt, M. M. Leggatt and B. J. Miflin Protein metabolism in developing endosperms of high-lysine and normal barley, Cereal Chem. 56: 110 (1979).

    CAS  Google Scholar 

  18. B. J. Miflin and P. R. Shewry, Seed storage proteins: genetics, synthesis, accumulation and protein quality. in: “Nitrogen and Carbon Metabolism”, J. D. Bewley ed., Martin Nijhoff, The Hague (1981).

    Google Scholar 

  19. P. R. Shewry, J. Franklin, S. Parmar, S. Smith and B. J. Miflin, The effects of sulphur starvation on the amino acid and protein composition of barley grain, J. Cereal Sei. (in press).

    Google Scholar 

  20. K-E. Karlsson, Linkage studies in a gene for high lysine content in Risø barley mutant 1508, Barley Genet. Newslett. 7:40 (1977).

    Google Scholar 

  21. J. Ingversen, B. Køie and H. Doll Induced seed protein mutant of barley. Experimentia 29: 1151 (1973).

    Article  CAS  Google Scholar 

  22. B. J. Miflin and P. R. Shewry, The synthesis of proteins in normal and high lysine barley seeds, in: “Recent Advances in the Biochemistry of Cereals”, D. Laidman and R. G. Wyn Jones, eds. Academic Press, London (1979).

    Google Scholar 

  23. H. Doll, A nearly non-functional mutant allele of the storage protein locus Hor 2 in barley, Hereditas 93:217 (1980).

    Article  CAS  Google Scholar 

  24. P. R. Shewry, J. M. Field, M. A. Kirkman, A. J. Faulks and B. J. Miflin, The extraction solubility and characterization of two groups of barley storage polypeptides, J. exp. Bot. 31:393, (1980).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, UK

    B. J. Miflin, S. Rahman, M. Kreis, B. G. Forde, L. Blanco & P. R. Shewry

Authors
  1. B. J. Miflin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kreis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. G. Forde
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Blanco
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. R. Shewry
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Genetics and Microbiology, University of Pavia, Pavia, Italy

    Orio Ciferri

  2. University of Georgia, Athens, Georgia, USA

    Leon Dure III

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Plenum Press, New York

About this chapter

Cite this chapter

Miflin, B.J., Rahman, S., Kreis, M., Forde, B.G., Blanco, L., Shewry, P.R. (1983). The Hordeins of Barley: Developmentally and Nutritionally Regulated Multigene Families of Storage Proteins. In: Ciferri, O., Dure, L. (eds) Structure and Function of Plant Genomes. NATO Advanced Science Institutes Series, vol 63. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4538-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-4538-1_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4540-4

  • Online ISBN: 978-1-4684-4538-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation