Photobiological Techniques

1. Front Matter

   Pages i-xiv

   PDF

2. Photophysics

   1. The Nature of the Light Field in Biological Media

      • Brian C. Wilson

      Pages 1-18

   2. Measurement of Fluoresecence Decays

      • Alessandra Andreoni

      Pages 19-35

   3. A Non-Invasive Technique for in Vivo Pharmacokinetic Measurements Using Optical Fiber Spectrofluorimetry

      • David A. Russell

      Pages 37-43

3. Photochemistry

   1. Quantum Yield of a Photochemical Reaction

      • Roy H. Pottier, David A. Russell

      Pages 45-57

   2. Photochemistry and Photobiology of Tetrapyrrole Pigments

      • Antony F. McDonagh

      Pages 59-76

   3. The Flavin-EDTA System in the Study of Photocatalytic Redox Reactions

      • Aurelio Serrano

      Pages 77-84

4. Photosensitization

   1. Photosensitization: Reaction Pathways

      • Johan E. Van Lier

      Pages 85-98

   2. Membrane Photomodification

      • Dennis Paul Valenzeno

      Pages 99-115

   3. Spectroscopic Determination of Hematoporphyrin-Membrane Partition Parameters

      • Eitan Gross

      Pages 117-126

5. Photosynthesis

   1. Near Infra-Red Flash Absorption: A Tool for Studying Photosynthesis

      • Paul Mathis, Danièle Thibodeau

      Pages 127-145

   2. Chlorophyll Fluorescence Transients in Chloroplasts and Leaves

      • Jean-Marc Ducruet, Paul Mathis

      Pages 147-163

6. UV Effects

   1. Response to Ultraviolet Radiation in a Simple Eukaryote (Yeast): Genetic Control and Biological Consequences

      • Dietrich Averbeck, Ethel Moustacchi

      Pages 165-186

7. Environmental Photobiology

   1. Isolation and Biological Activity of Plant Derived Phototoxins

      • John Thor Arnason, Robin J. Marles, Richard R. Aucoin

      Pages 187-196

8. Vision

   1. Visual Pigments: Absorbance Spectra and Photoproducts

      • James K. Bowmaker

      Pages 197-212

   2. Measurement of Retinal Function Using the Electroretinogram

      • Ron Douglas

      Pages 213-230

9. Photomorphogenesis

   1. Photomorphogenic Responses to UV Light: Involvement of Phytochrome and UV Photoreceptors

      • Bartolomeo Lercari

      Pages 231-248

10. Photomovement

    1. Photomovement

       • Wilhelm Nultsch, Jürgen Pfau

       Pages 249-277

11. Chronobiology

    1. Effects of Light on Circadian Rhythms in Plants and Animals

       • Wilhelm Nultsch, Gerhard Heldmaier, Glenn Weagle

       Pages 279-295

12. Bioluminescence

    1. Bioluminescence: Biochemistry for Fun and Profit

       • John Lee

       Pages 297-321

The fIrst edition of the Science 0/ Photobiology edited by Kendric C. Smith (plenum Press, 1977) was a comprehensive textbook of photobiology, devoting a chapter to each of the subdisciplines of the fIeld. At the end of many of these chapters there were brief descriptions of simple experiments that students could perform to demonstrate the principles discussed. In the succeeding years some photobiologists felt that a more complete publica­ tion of experiments in photobiology would be a useful teaching tool. Thus, in the 1980s the American Society for Photobiology (ASP) attempted to produce a laboratory manual in photobiology. Cognizant of these efforts, Kendric Smith elected to publish the second edition of The Science o/Photobiology (1989) without experiments; anticipating the comple­ tion of the ASP laboratory manual. Unfortunately, the initial ASP efforts met with limited success, and several years were to pass before a photobiology laboratory manual became a reality. One of the major stumbling blocks to production of an accurate and reliable laboratory manual was the requirement that the experiments be tested, not just by the author who is familiar with the techniques, but by students who may be quite new to photobiology. How could this be accomplished with limited resources? Many ideas were considered and discarded, before a workable solution was found. The catalyst that enabled the careful screening of all experiments in this book was a NATO Advanced Study Institute (ASI) devoted entirely to this purpose.

• Fortuna
• Phytochrome
• biochemistry
• chemistry
• reaction

• The University of Kansas Medical Center, Kansas City, USA

  Dennis Paul Valenzeno

• The Royal Military College of Canada, Kingston, Canada

  Roy H. Pottier

• CEN Saclay, Gif-sur-Yvette, France

  Paul Mathis

• The City University, London, UK

  Ron H. Douglas

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