Abstract
Immobilization of lipases is gaining much attention these days due to the wide variety of reactions catalyzed by them. Moreover, the lipases play an important role in biodiesel production. In this study, lipase from Burkholderia cepacia was first cross-linked with glutaraldehyde followed by entrapment into hybrid matrix of equal proportions of alginate and κ-carrageenan natural polymers. The immobilized lipase gave promising results with stability parameters like pH, temperature, solvent, storage, enzyme leakage, and hydrolytic activity. A significant reduction of 65.76 % of enzyme leakage was obtained with this immobilized lipase. Moreover, a 100 % yield of biodiesel was produced from crude Jatropha curcas oil using this immobilized lipase.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Cao, L. Q., Langen, V. L., & Sheldon, R. A. (2003). Immobilized enzymes: carrier-bound or carrier-free? Current Opinions in Biotechnology, 14, 387–394.
Jegannathan, K. R., Sariah, A., Denis, P., Chan, E. S., & Ravindra, P. (2008). Production of biodiesel using immobilized lipase—a critical review. Critical Reviews in Biotechnology, 28, 253–264.
Rahmath, A., Chan, E. S., & Ravindra, P. (2011). Biodiesel production from Jatropha curcas: a critical review. Critical Reviews in Biotechnology, 31, 53–64.
Sahin, F., Demirel, G., & Tumturk, H. (2005). A novel matrix for the immobilization of acetylcholinesteras. International Journal of Biological Macromolecules, 37, 148–153.
Xavier, M. F., Hector, R. R., Hugo, S. G., Charles, G. H., & Clyde, H. A. (1990). Immobilized lipase reactors for modification of fats and oils—a review. Journal of American Oil Chemists Society, 67, 890–910.
Acknowledgments
Authors would like to thank the University Malaysia Sabah and Ministry of Science, Technology and Innovation (MOSTI) for funding this project, FGRG0244-TK-1/2010.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this chapter
Cite this chapter
Abdulla, R., Ravindra, P. (2013). Cross-linked Lipase in Hybrid Matrix for Biodiesel Production from Crude Jatropha Curcas Oil. In: Pogaku, R., Bono, A., Chu, C. (eds) Developments in Sustainable Chemical and Bioprocess Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-6208-8_25
Download citation
DOI: https://doi.org/10.1007/978-1-4614-6208-8_25
Published:
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4614-6207-1
Online ISBN: 978-1-4614-6208-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)