Abstract
Fluorescent semiconductor quantum dots (Qdots) hold great potential for both ex vivo and in vivo molecular imaging. Traditional imaging with single-photon confocal microscopy and organic fluorophores poses several challenges for the visualization of vascular tissue, including autofluorescence, fluorophore crosstalk, and photobleaching. We will review a recently developed immunohistochemical (IHC) en face method that employs quantum dot bioconjugates and two-photon excitation laser scanning microscopy (TPELSM). These techniques improve contrast resolution and allow detailed cellular structures to be imaged without the common problem of vascular autofluorescence. It is especially useful for multicolor profiling of endothelial structures with only one excitation wavelength. Moreover, this is also a promising technique for mapping the expression of endothelial proteins and their relationship to flow dynamics. In addition, a more reliable fluorescence quantitation of endothelial signals will benefit the ex vivo testing of different interventions. By providing a bridge between in vitro and in vivo studies, this method may facilitate the development of novel drugs and specific multimodal molecular imaging agents.
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Ferrara, D.E., Glaus, C., Taylor, W.R. (2008). Targeting Vascular Epitopes Using Quantum Dots. In: Bulte, J.W., Modo, M.M. (eds) Nanoparticles in Biomedical Imaging. Fundamental Biomedical Technologies, vol 102. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72027-2_21
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DOI: https://doi.org/10.1007/978-0-387-72027-2_21
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