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Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells

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Human Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1307))

Abstract

The neural crest (NC) is a transient population of multipotent cells giving rise to the peripheral nervous system, skin pigmentation, heart, and facial mesenchyme. The broad cell fate potential of NC makes it an attractive cell fate to derive from human pluripotent stem cells (hPSCs) for exploring embryonic development, modeling disease, and generating cells for transplantation. Here, we discuss recent publications and methods for efficiently differentiating hPSCs into NC. We also provide methods to direct NC into two different terminal fates: melanocytes and sensory neurons.

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Acknowledgments

M.J.T. and L.S. are supported by The Starr Foundation and NYSTEM. Protocol development was supported in part by grants from NYSTEM (CO26446 and CO26447 to L.S, C026399 to S.M.C., C024175 to L.S. and M.J.T.), the Joanna M. Nicolay Melanoma Foundation (Y.M.), and in part through NS066390 from National Institute of Neurological Disorders and Stroke/US National Institutes of Health (NS066390 to L.S.). G.L. is supported by the Robertson Investigator Award of the New York Stem Cell Foundation and from the Maryland Stem Cell Research Fund (TEDCO).

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Authors and Affiliations

  1. Developmental Biology Program, Center for Stem Cell Biology, Sloan-Kettering Institute, 1275 York Ave, New York, NY, 10065, USA

    Stuart M. Chambers & Yvonne Mica

  2. Life Technologies, 5791 Van Allen Way, Carlsbad, CA, 92008, USA

    Yvonne Mica

  3. Department of Neurology and Neuroscience, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Gabsang Lee

  4. Developmental Biology Program, Department of Neurosurgery, Center for Stem Cell Biology, Sloan-Kettering Institute, 1275 York Ave, New York, NY, 10065, USA

    Lorenz Studer

  5. Developmental Biology Program, SKI Stem Cell Research Facility, Center for Stem Cell Biology, Sloan-Kettering Institute, 1275 York Ave, New York, NY, 10065, USA

    Mark J. Tomishima

Authors
  1. Stuart M. Chambers
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  2. Yvonne Mica
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  3. Gabsang Lee
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  4. Lorenz Studer
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  5. Mark J. Tomishima
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Corresponding author

Correspondence to Mark J. Tomishima .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2013 Springer Science+Business Media New York

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Chambers, S.M., Mica, Y., Lee, G., Studer, L., Tomishima, M.J. (2013). Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1307. Humana Press, New York, NY. https://doi.org/10.1007/7651_2013_59

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  • DOI: https://doi.org/10.1007/7651_2013_59

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2667-1

  • Online ISBN: 978-1-4939-2668-8

  • eBook Packages: Springer Protocols

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