Abstract
This study seeks to determine the pathogens in respiratory specimens and blood serum obtained from children who present with community acquired pneumonia (CAP) diagnosed on the basis of clinical and radiological evidence. The study group consisted of 46 hospitalized children aged 1–11 years. The material for research consisted of pharyngeal swabs and samples of blood serum. One hundred and thirty eight pharyngeal swabs were examined for the presence of C. pneumoniae antigen, C. pneumoniae DNA, and for typical pathogens. C. pneumoniae DNA was detected in pharyngeal swabs with nested PCR. Classical microbiological culture was used for detection of typical bacteria. ELISA test were used for detection anti-C. pneumoniae and anti-M. pneumoniae antibodies in the serum. C. pneumoniae DNA was identified in 10.9% of children. Positive culture for typical pathogens was observed in 8.7% of children. Specific anti-C. pneumoniae IgM antibodies were found in 8.7% of children, and IgG and IgA antibodies in 1 child each. Specific anti-M. pneumoniae IgG antibodies were found in 13.1% of children and IgM antibodies in 1 child. We conclude that the underlying bacterial etiology of CAP is rather rarely conclusively confirmed in children. Nonetheless, determining the etiology of CAP is essential for the choice of treatment to optimize the use and effectiveness of antimicrobials and to avoid adverse effect. Due to considerable variations in the power of detection of the type of atypical bacteria causing CAP, the search for the optimum diagnostic methods continues.
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Acknowledgments
Supported by grant E090.16.065 from the Medical University in Wroclaw, Poland.
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The authors declare no conflicts of interest in relations to this article.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study protocols were accepted by the Ethics Committee of the Medical University of Wroclaw, Poland.
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Written informed consent was obtained from all individual participants and/or their legal guardians for participation in the study.
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Jama-Kmiecik, A., Frej-Mądrzak, M., Sarowska, J., Teryks-Wołyniec, D., Skiba, A., Choroszy-Król, I. (2019). Atypical and Typical Bacteria in Children with Community Acquired Pneumonia. In: Pokorski, M. (eds) Advances in Pulmonary Medicine: Research and Innovations. Advances in Experimental Medicine and Biology(), vol 1160. Springer, Cham. https://doi.org/10.1007/5584_2019_377
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DOI: https://doi.org/10.1007/5584_2019_377
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