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Mammalian Cell-Based Sensor System

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Whole Cell Sensing Systems I

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 117))

Abstract

Use of living cells or cellular components in biosensors is receiving increased attention and opens a whole new area of functional diagnostics. The term “mammalian cell-based biosensor” is designated to biosensors utilizing mammalian cells as the biorecognition element. Cell-based assays, such as high-throughput screening (HTS) or cytotoxicity testing, have already emerged as dependable and promising approaches to measure the functionality or toxicity of a compound (in case of HTS); or to probe the presence of pathogenic or toxigenic entities in clinical, environmental, or food samples. External stimuli or changes in cellular microenvironment sometimes perturb the “normal” physiological activities of mammalian cells, thus allowing CBBs to screen, monitor, and measure the analyte-induced changes. The advantage of CBBs is that they can report the presence or absence of active components, such as live pathogens or active toxins. In some cases, mammalian cells or plasma membranes are used as electrical capacitors and cell–cell and cell–substrate contact is measured via conductivity or electrical impedance. In addition, cytopathogenicity or cytotoxicity induced by pathogens or toxins resulting in apoptosis or necrosis could be measured via optical devices using fluorescence or luminescence. This chapter focuses mainly on the type and applications of different mammalian cell-based sensor systems.

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Acknowledgments

Research in the authors’ laboratory was supported through a cooperative agreement with the Agricultural Research Service of the US Department of Agriculture (USDA) project number 1935-42000-035, the Center for Food Safety and Engineering at Purdue University, and USDA-NRI (2005-35603-16338). BF is supported by the USDA National Needs Fellowship.

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  1. Laboratory of Food Microbiology & Immunochemistry, Department of Food & Animal Sciences, Alabama A&M University, Normal, AL, 35762, USA

    Pratik Banerjee & Briana Franz

  2. Molecular Food Microbiology Laboratory, Center for Food Safety Engineering, Purdue University, West Lafayette, IN, 47907, USA

    Arun K. Bhunia

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  1. Pratik Banerjee
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Correspondence to Arun K. Bhunia .

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  1. , Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel

    Shimshon Belkin

  2. , College of Life Sciences & Biotechnology, Korea University, Amam-dong 5-ga, Seoul, 136-701, Korea, Republic of (South Korea)

    Man Bock Gu

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Banerjee, P., Franz, B., Bhunia, A.K. (2010). Mammalian Cell-Based Sensor System. In: Belkin, S., Gu, M. (eds) Whole Cell Sensing Systems I. Advances in Biochemical Engineering / Biotechnology, vol 117. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_21

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