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Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

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Abstract

Yellow mosaic disease, caused by a whitefly transmitted New World Begomovirus, named Corchorus golden mosaic virus (CoGMV), is emerging as a serious biotic constraint for jute fibre production in Asia. For rapid and sensitive diagnosis of the Begomovirus associated with this disease, a non-radiolabelled diagnostic probe, developed against the DNA A component of the east Indian isolate of CoGMV, detected the presence of the virus in infected plants and viruliferous whiteflies following Southern hybridization and nucleic acid spot hybridization tests. Presence of the virus was also confirmed when polymerase chain reaction amplification was performed using virus-specific primers on DNA templates isolated from infected plants and viruliferous whiteflies.

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Acknowledgments

We acknowledge Director, Central Research Institute for Jute and Allied Fibres for providing the infrastructural support in the present investigation. The first author is also grateful to Indian Council of Agricultural Research, New Delhi for providing financial assistance during the tenure of which this work was carried out.

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Correspondence to Raju Ghosh.

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Ghosh, R., Palit, P., Paul, S. et al. Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis). Indian J. Virol. 23, 70–74 (2012). https://doi.org/10.1007/s13337-012-0062-7

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  • DOI: https://doi.org/10.1007/s13337-012-0062-7

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