Skip to main content

Advertisement

SpringerLink
Log in

Effects of Selenium and Light Wavelengths on Liquid Culture of Cordyceps militaris Link

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

To investigate the effects of selenium and light wavelengths on the growth of liquid-cultured Cordyceps militaris and the main active components’ accumulation, culture conditions as selenium selenite concentrations and light of different wavelengths were studied. The results are: adenosine accumulation proved to be significantly selenium dependent (R 2 = 0.9403) and cordycepin contents were determined to be not significantly selenium dependent (R 2 = 0.3845) but significantly enhanced by selenium except for 20 ppm; there were significant differences in cordycepin contents, adenosine contents, and mycelium growth caused by light wavelengths: cordycepin, blue light > pink light > daylight, darkness, red light; adenosine, red light > pink light, darkness, daylight, blue light; and mycelium growth, red light > pink light, darkness, daylight > blue light. In conclusion, light wavelength had a significant influence on production of mycelia, adenosine, and cordycepin, so lightening wavelength should be changed according to target products in the liquid culture of C. militaris.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Das, S. K., Masuda, M., Sakurai, A., & Sakakibara, M. (2010). Medicinal uses of the mushroom Cordyceps militaris: current state and prospects. Fitoterapia, 81, 961–968.

    Article  Google Scholar 

  2. Khan, M. A., Tania, M., Zhang, D., & Chen, H. (2010). Cordyceps mushroom: a potent anticancer nutraceutical. The Open Nutraceuticals Journal, 8, 179–183.

    Article  Google Scholar 

  3. Zhang, A., Lu, J., Zhang, N., Zheng, D., Zhang, G., & Teng, L. (2010). Extraction, purification and anti-tumor activity of polysaccharide from mycelium of mutant Cordyceps militaris. Chemical Research in Chinese Universities, 26, 798–802.

    CAS  Google Scholar 

  4. Chen, C., Luo, S. S., Li, Y., Sun, Y. J., & Zhang, C. K. (2004). Study on antioxidant activity of three Cordyceps sp. by chemiluminescence. Shanghai J Traditional Chinese Medicine, 38, 53–55. in Chinese.

    Google Scholar 

  5. Shih, I. L., Tsai, K. L., & Hsieh, C. (2007). Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris. Biochemical Engineering Journal, 33, 193–201.

    Article  CAS  Google Scholar 

  6. Ahn, Y. J., Park, S. J., Lee, S. G., Shin, S. C., & Choi, D. H. (2000). Cordycepin: selective growth inhibitor derived from liquid culture of Cordycceps militaris against Clostridium spp. Journal of Agricultural and Food Chemistry, 48, 2744–2748.

    Article  CAS  Google Scholar 

  7. Kodama, E. N., McCaffrey, R. P., Yusa, K., & Mitsuya, H. (2000). Antileukemic activity and mechanism of action of cordycepin against terminal deoxynucleotidyl transferase-positive (TdT+) leukemic cells. Biochemical Pharmacology, 59, 273–281.

    Article  CAS  Google Scholar 

  8. Choi, S. B., Park, C. H., Choi, M. K., Jun, D. W., & Park, S. (2004). Improvement of insulin resistance and insulin secretion by water extracts of Cordyceps militaris, Phellinus linteus, and Paecilomyces tenuipes in 90% pancreatectomized rats. Bioscience, Biotechnology, and Biochemistry, 68, 2257–2264.

    Article  CAS  Google Scholar 

  9. Jung, K., Kim, I. H., & Han, D. (2004). Effect of medicinal plant extracts on forced swimming capacity in mice. Journal of Ethnopharmacology, 93, 75–81.

    Article  Google Scholar 

  10. Won, S. Y., & Park, E. H. (2005). Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris. Journal of Ethnopharmacology, 96, 555–561.

    Article  Google Scholar 

  11. The State Pharmacopoeia Commission of China (2005) Pharmacopoeia of China (pp. 75, vol I). Beijing: Chemical Industry Press.

  12. Wong, Y. Y., Moon, A., Duffin, R., Barthet-Barateig, A., Meijer, H. A., Clemens, M. J., & Moor, C. H. (2010). Cordycepin inhibits protein synthesis and cell adhesion through effects on signal transduction. The Journal of Biological Chemistry, 285, 2610–2621.

    Article  CAS  Google Scholar 

  13. Almagro F. L., Sabater-Jara, A. B., Belchí-Navarro, S., Fernández-Pérez, F., Bru, R., Pedreño, M. A. (2011). Effect of UV light on secondary metabolite biosynthesis in plant cell cultures elicited with cyclodextrins and methyl jasmonate. In: Plants and environment (pp.115-136). Charlotte: InTech

  14. Lin, C. (2002). Blue light receptors and signal transduction. The Plant Cell, 14, 207–225.

    Google Scholar 

  15. Idnurm, A., Rodríguez-Romero, J., Corrochano, L. M., Sanz, C., Iturriaga, E. A., Eslava, A. P., & Heitman, J. (2006). The phycomyces madA gene encodes a blue-light photoreceptor for phototropism and other light responses. Proceedings of the National Academy of Sciences, 103, 4546–4551.

    Article  CAS  Google Scholar 

  16. Cheng, P., Yang, Y., Wang, L., He, Q., & Liu, Y. (2002). WHITE COLLAR-1, a multifunctional Neurospora protein involved in the circadian feedback loops, light sensing, and transcription repression of wc-2. The Journal of Biological Chemistry, 278, 3801–3808.

    Article  Google Scholar 

  17. Velayos, A., Blasco, J. L., Alvarez, M. I., & Iturriaga, E. A. (2000). Blue-light regulation of phytoene dehydrogenase (carB) gene expression in Mucor circinelloides. Planta, 210, 938–946.

    Article  CAS  Google Scholar 

  18. Tara, F., Rayman, M. P., & Boskabadi, H. (2010). Prooxidant-antioxidant balance in pregnancy: a randomized double-blind placebo-controlled trial of selenium supplementation. Journal of Perinatal Medicine, 38, 473–478.

    Article  CAS  Google Scholar 

  19. Ramoutar, R. R., & Brumaghim, J. L. (2010). Antioxidant and anticancer properties and mechanisms of inorganic selenium, oxo-sulfur, and oxo-selenium compounds. Cell Biochemistry and Biophysics, 58, 1–23.

    Article  CAS  Google Scholar 

  20. Silva, I. C. M., Ribeiro, A. M. L., Canal, C. W., Trevizan, L., Macagnan, M., Gonçalves, T. A., Hlavac, N. R. C., de Almeida, L. L., & Pereira, R. A. (2010). The impact of organic and inorganic selenium on the immune system of growing broilers submitted to immune stimulation and heat stress. Brazilian Journal of Poultry Science, 4, 247–254.

    Google Scholar 

  21. Yu, R. M., Yang, W., Song, L. Y., Yan, C. Y., Zhang, Z., & Zhao, Y. (2007). Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordycceps militaris. Carbohydrate Polymers, 70, 430–436.

    Article  CAS  Google Scholar 

  22. Chun, J. Y., Nadiminty, N., Lee, S. O., Onate, S. A., Lou, W., & Gao, A. C. (2006). Mechanisms of selenium down-regulation of androgen receptor signaling in prostate cancer. Molecular Cancer Therapeutics, 5, 913–918.

    Article  CAS  Google Scholar 

  23. Uden, P. C., Boakye, H. T., Kahakachchi, C., Hafezi, R., Nolibos, P., Block, E., Johnson, S., & Tyson, J. F. (2003). Element selective characterization of stability and reactivity of selenium species in selenized yeast. Journal of Analytical Atomic Spectrometry, 18, 1–10.

    Article  Google Scholar 

  24. Dong, J. Z., Lei, C., Ai, X. R., & Wang, Y. (2012). Selenium enrichment on Cordyceps militaris Link and analysis on its main active components. Applied Biochemistry and Biotechnology, 166, 1215–1224.

    Google Scholar 

  25. Xing, L., Cohen, J. D., & Gardner, G. (2011). Low-fluence red light increases the transport and biosynthesis of auxin. Plant Physiology, 157, 891–904.

    Article  Google Scholar 

  26. Denault, D. L. (2001). WC-2 mediates WC1-FRQ interaction within the PAS protein-linked circadian feedback loop of Neurospora. The EMBO Journal, 20, 109–117.

    Article  CAS  Google Scholar 

  27. Smirnov, D.A., Shcherba, V.V., Bisko, N.A., Poyedinok, N.L. (2009). Some biologically active substances from a mycelial biomass of medicinal caterpillar fungus Cordyceps sinensis (Berk.) Sacc. (Ascomycetes). International Journal of Medicinal Mushrooms, 1, 69-76.

    Google Scholar 

  28. Poyedinok, N.L., Mykhailova, O.B., Shcherba, V.V., Buchalo, A.S., Negriyko, A.M. (2008). Light regulation of growth and biosynthetic activity of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae), in pure culture. International Journal of Medicinal Mushrooms, 4, 369-378.

  29. Puchkova, T. A., Babitskaya, V. G., Scherba, V., Bisko, N. A., & Poyedinok, N. L. (2010). Polysaccharides of medicinal caterpillar fungus, Cordyceps militaris Link (Ascomycetes): production and composition. International Journal of Medicinal Mushrooms, 4, 419–425.

    Article  Google Scholar 

  30. Kredich, N. M., & Guarino, A. J. (1961). Studies on the biosynthesis of cordycepin. Biochimica et Biophysica Acta, 47, 529–534.

    Article  CAS  Google Scholar 

  31. Ulusu, N. N., & Turan, B. (2005). Beneficial effects of selenium on some enzymes of diabetic rat heart. Biological Trace Element Research, 3, 207–215.

    Article  Google Scholar 

Download references

Acknowledgments

This research was partially funded by the Ethnological Affair Committee Foundation of China (Grant No. 10HB02) and by the Doctor Research Project (498012) of Hubei University for Nationalities. We are grateful to anonymous reviewers and scientific editor for the critical review and valuable suggestions.

Author information

Authors and Affiliations

  1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, School of Biological Science and Technology, Hubei University for Nationalities, Enshi, 445000, China

    J. Z. Dong, M. R. Liu, C. Lei & X. J. Zheng

  2. Key Laboratory of Pant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China

    Y. Wang

Authors
  1. J. Z. Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. R. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. X. J. Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to J. Z. Dong or Y. Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dong, J.Z., Liu, M.R., Lei, C. et al. Effects of Selenium and Light Wavelengths on Liquid Culture of Cordyceps militaris Link. Appl Biochem Biotechnol 166, 2030–2036 (2012). https://doi.org/10.1007/s12010-012-9628-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9628-5

Keywords

Search

Navigation