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Agrobacterium-mediated transformation of Lupinus mutabilis L. using shoot apical explants

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Abstract

A procedure for regenerating plants of Lupinus mutabilis from shoot apices, from which the leaf primordia and initial cell layer(s) of the apical meristem were removed, has been used to generate transgenic plants following Agrobacterium tumefaciens-mediated gene delivery. Transformation competent cells, from which buds developed, were located at the periphery of the apical meristem. Kanamycin resistant plants were obtained which expressed β-glucuronidase activity. Integration of the neomycin phosphotransferase II and β-glucuronidase genes into the genomes of transgenic plants was confirmed by non-radioactive DNA-DNA hybridisation. This is the first report of the generation of transgenic plants in L. mutabilis.

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Abbreviations

BAP:

6-benzylamino purine

bar :

bialaphos (phosphinothricin) resistance gene

CPPU:

N-(2-chloro-4-pyridyl)-N′-phenylurea

DAS:

Double Antibody Sandwich

GUS:

β-glucuronidase

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog (1962)

4-MU:

4-methylumbelliferyl glucuronide

NAA:

α-naphthaleneacetic acid

NPTII:

neomycin phosphotransferase

X-Gluc:

5-bromo-4-chloro-3-indolyl-β-D-glucuronide

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Author notes

  1. Mehmet Babaoglu

    Present address: Faculty of Agriculture, University of Selçuk, Kampüs, Konya, Turkey

Authors and Affiliations

  1. Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    Mehmet Babaoglu, Matthew S. McCabe, J. Brian Power & Michael R. Davey

Authors
  1. Mehmet Babaoglu
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  2. Matthew S. McCabe
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  3. J. Brian Power
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  4. Michael R. Davey
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Babaoglu, M., McCabe, M.S., Power, J.B. et al. Agrobacterium-mediated transformation of Lupinus mutabilis L. using shoot apical explants. Acta Physiol Plant 22, 111–119 (2000). https://doi.org/10.1007/s11738-000-0064-8

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  • DOI: https://doi.org/10.1007/s11738-000-0064-8

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