Skip to main content
SpringerLink
Log in

Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”

  • IAPB March/April 07
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Using immature embryos and cotyledons as explants, a successful system to culture immature embryos and induce direct regeneration from cotyledons was established for Prunus mume “Xuemei”. For immature embryo culture, a high frequency of plantlet formation (89.5%) from the embryonic axis was obtained using half-strength Murashige and Skoog (1/2 MS) medium supplemented with 13.2 μM 6-benzyladenine (BA) and 2.7 μM 1-naphthaleneacetic (NAA). Shoots formed directly from cotyledons with the embryo axis intact when explants were cultured on 1/2 MS medium containing 2.2 μM BA with different combinations of NAA (2.7, 5.4 μM) and indole-3-butyric acid (IBA) (0, 2.5, 5.0 μM). Better results were achieved when the embryonic axis was removed from the cotyledons and cultured on 1/2 MS medium supplement with 13.2 μM BA, 2.7 μM NAA or 2.2 μM BA, 2.2 μM thidiazuron (TDZ), and 2.7 μM NAA, respectively. Regenerated shoots were successfully rooted on 1/2 MS or Woody Plant medium (WPM) supplemented with 2.5–5.0 μM IBA. The effect of the embryonic axis, BA, and TDZ on cotyledon regeneration was investigated in detail. Rooted plantlets were transferred to soil successfully.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1.

Similar content being viewed by others

References

  • Antonelli, M. Regeneration from almond cotyledons: induction of proembryonal masses. Acta Hortic. 300: 255–259; 1991.

    Google Scholar 

  • Bhagwat, B.; David, L. W. In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) ‘Lapins’ and ‘Sweetheart’. Plant Cell Tissue Organ Cult. 78: 173–181; 2004.

    Article  CAS  Google Scholar 

  • Chen, J. Y. Prunus mume in China. Haikou, China: Hainan Publishing House; 1997.

    Google Scholar 

  • Declerck, V.; Korban, S. S. Influence of growth regulators and carbon sources on callus induction, growth and morphogenesis from leaf tissues of peach (Prunus persica L. Batsch). J. Hort. Sci. 71:49–55; 1996.

    CAS  Google Scholar 

  • Dogasaki, C.; Kakuno, Y.; Honda, M.; Takada, N.; Maruyama, T.; Nishijima, M.; Adachi, Y.; Ohno, N.; Yadomae, T.; Miyazaki, T. Contribution to immunochemical analysis of polysaccharides in medicinal plants. Drug Design Rev. 1: 153–159; 2004.

    Article  CAS  Google Scholar 

  • Escalettes, V.; Dosba, F. In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Sci. 90: 201–209; 1993.

    Article  Google Scholar 

  • Goffreda, J. C.; Scopel, A. L.; Fiola, J. A. Indole butyric acid induces regeneration of phenotypically normal apricot (Prunus armeniaca L.) plants from immature embryos. Plant Growth Regul. 17: 41–46; 1995.

    CAS  Google Scholar 

  • Han, J. S.; Oh, D. G.; Mok, I. G.; Park, H. G.; Kim, C. K. Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl). Plant Cell Rep. 23: 291–296; 2004.

    Article  PubMed  CAS  Google Scholar 

  • Harada, H. H.; Murai, Y. Micropropagation of Prunus mume. Plant Cell Tissue Organ Cult. 46: 265–267; 1996.

    Article  CAS  Google Scholar 

  • Hokanson, K. E.; Pooler, M. R. Regeneration of ornamental cherry (Prunus) taxa from mature stored seed. HortScience. 35: 745–748; 2000.

    CAS  Google Scholar 

  • Katoka, I. Interspecific hybridization between Microcerasus and Prunus spp. J. Jpn. Soc. Hort. Sci. 4: 398–407; 1988.

    Google Scholar 

  • Kouider, M.; Korban, S. S.; Skirvin, R. M.; Chu, M. N. Influence of embryonic dominance and polarity on adventitious shoot formation from apple cotyledons in vitro. J. Am. Soc. Hort. Sci. 109: 381–385; 1984.

    Google Scholar 

  • Lane, W. D.; Cossio, F. Adventitious shoots from cotyledons of immature cherry and apricot embryos. Can. J. Plant Sci. 66: 953–959; 1986.

    Article  CAS  Google Scholar 

  • Lloyd, G. B.; McCown, B. H. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant. Prop. Soc. 30: 421–427; 1980.

    Google Scholar 

  • Mante, S. R.; Scorza, R.; Cordts, J. M. Plant regeneration from cotyledons of Prunus persica, Prunus domestica and Prunus cerasus. Plant Cell Tissue Organ Cult. 19: 1–11; 1989.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.

    Article  CAS  Google Scholar 

  • Pellegrineschi, A.; Fatokun, C. A.; Thottappilly, G.; Adepoju, A. Cowpea embryo rescue.1. Influence of culture medium composition on plant recovery from isolated immature embryos. Plant Cell Rep. 17: 133–138; 1997.

    Article  CAS  Google Scholar 

  • Pooler, M. R.; Scorza, R. Regeneration of peach (Prunus persica (L.) Batsch) rootstock cultivars from cotyledons of mature stored seed. HortScience. 30: 355–356; 1995.

    Google Scholar 

  • Salajova, T.; Salaj, J. Somatic embryogenesis and plantlet regeneration from cotyledon explants isolated from embryos and seedlings of hybrid fir. J. Plant Physiol. 158: 747–755; 2001.

    Article  CAS  Google Scholar 

  • Simon, H.; Raharjo, T.; Litz, R. E. Micrografting and ex vitro grafting for somatic embryo rescue and plantrecovery in avocado (Persea americana). Plant Cell Tissue Organ Cult. 82: 1–9; 2005.

    Article  Google Scholar 

  • Singh, A. K; Suresh, C. Somatic embryogenesis and plant regeneration from cotyledon explants of a timber-yielding leguminous tree, Dalbergia sissoo Roxb. J. Plant Physiol. 160: 415–421; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Viloria, Z.; Grosser, J. W.; Bracho, B. Immature embryo rescue, culture and seedling development of acid citrus fruit derived from interploid hybridization. Plant Cell Tissue Organ Cult. 82: 159–167; 2005.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank all the colleagues in our lab for constructive discussion and technical support, and are also grateful to all the staff of Wuhan P. mume garden, China for providing experimental materials.

Author information

Authors and Affiliations

  1. Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    G. G Ning, S. P Bai, M. Z Bao & L. Liu

Authors
  1. G. G Ning
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. P Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Z Bao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Z Bao.

Additional information

Editor: F. Hammerschlag

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ning, G.G., Bai, S.P., Bao, M.Z. et al. Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”. In Vitro Cell.Dev.Biol.-Plant 43, 95–100 (2007). https://doi.org/10.1007/s11627-007-9035-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-007-9035-8

Keywords

Search

Navigation