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Thidiazuron-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.)

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Summary

Improved in vitro tissue culture systems are needed to facilitate the application of recombinant DNA technology to the improvement of sugar beet germplasm. The effects of N 6-benzyladenine (BA) and thidiazuron (TDZ) pretreatment on adventitious shoot and somatic embryogenesis regeneration were evaluated in a range of sugar beet breeding lines and commercial varieties. Petiole explants showed higher frequencies of direct adventitious shoot formation and produced more shoots per explant than leaf lamina explants. TDZ was more effective than BA for the promotion of shoot formation. The optimal TDZ concentrations were 2.3–4.6 μM for the induction of adventitious shoot regeneration. Direct somatic embryogenesis from intact seedlings could be induced by either BA or TDZ. TDZ-induced somatic embryogenesis occurred on the lower surface of cotyledons at concentrations of 0.5–2μM and was less genotype-dependent than with Ba. A high frequency of callus induction could be obtained from seedlings and leaf explants, but only a few of the calluses derived from leaf explants could regenerate to plants via indirect somatic embryogenesis. These results demonstrated that TDZ could prove to be a more effective cytokinin for in vitro culture of sugar beet than BA. Rapid and efficient regeneration of plants using TDZ may provide a route for the production of transgenic sugar beet following Agrobacterium-mediated transformation.

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References

  • Atanassov, A. I. Sugar beet. In: Evans, D. A.; Sharp, W. R.; Ammirato, P. V.; eds. Handbook of plant cell culture, vol. 4. New York: Macmillan; 1986;652–680.

    Google Scholar 

  • Brandstatter, J.; Kieber, J. J. Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinins in Arabidopsis. Plant Cell 10:1009–1019; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Capelle, S. C.; Mok, D. W.; Kirchner, S. C.; Mok, M. C. Effects of thidiazuron on cytokinin autonomy and the metabolism of N-γ′-isopentenyl (8-14C) adenosine in callus tissue of Phaseolus lunatus L. Plant. Physiol. 73:796–802; 1983.

    PubMed  CAS  Google Scholar 

  • Detrez, C.; Tetu, T.; Sangwan, R. S.; Sangwan-Norreel, B. S., Direct organogenesis from petiole and thin cell layer explants in sugar beet cultured in vitro. J. Exp. Bot. 39:917–926; 1988.

    Article  CAS  Google Scholar 

  • D'Hlluin, K.; Bossut, M.; Bonne, E.; Mazur, B.; Leemans, J.; Botterman, J. Transformation of sugarbeet (Beta vulgaris L.) and evaluation of herbicide resistance in transgenic plants. Bio/Technology 10:309–313; 1992.

    Article  Google Scholar 

  • Elliott, M. C.; Chen, D. F.; Fowler, M. R.; Kirby, M. J.; Kubalakova, M.; Scott, N. W.; Zhang, C. L.; Slater, A. Towards the perfect sugar beet via gene manipulation. Sugar Crops China I: 23–30; 1996.

    Google Scholar 

  • Gill, R.; Senaratna, T.; Saxena, P. K. Thidiazuron-induced somatic embryogenesis enhances the viability of hydrogel-encapsulated somatic embryos of geranium. J. Plant Physiol. 143:726–729; 1994.

    CAS  Google Scholar 

  • Gill, R.; Saxena, P. K. Somatic embryogenesis in Nicotiana tabacum L. Induction by thidiazuron of direct embryo differentiation from cultured leaf dises. Plant Cell Rep. 12:154–159; 1993.

    Article  Google Scholar 

  • Griever, T. M.; Gartland, K. M. A.; Elliott, M. C. Micropropagation of commercially important sugar beet cultivars. Plant Growth Reg. 21:15–18; 1997.

    Article  Google Scholar 

  • Hosemans, D.; Bossoutrot, D. Induction of haploid plants from in vitro culture of unpollinated beet ovules (Beta vulgaris L.). Z. Pflanz. 91:74–77; 1983.

    Google Scholar 

  • Huetteman, C. A.; Preece, J. E. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Organ Cult. 34:71–76; 1993.

    Article  Google Scholar 

  • Kaminek, M.; Armstrong, D. J. Genotypic variation in cytokinin oxidase from Phaseolus callus cultures. Plnat Physiol. 93:1530–1538; 1990.

    CAS  Google Scholar 

  • Kubalakova, M. Somatic embryogenesis and cytoplasmic sterility in Beta vulgaris L. var Saccharifera. Biol. Plant. 32:414–419; 1990.

    Google Scholar 

  • Levall, M. W.; Bornman, J. F. Selection in vitro for UV-tolerant sugarbeet (Beta vulgais) somaclones. Physiol. Planta 88:37–43; 1993.

    Article  CAS  Google Scholar 

  • Li, Z.; Jarret, R. L.; Pittman, R. N.; Demski, J. W. Shoot organogenesis from cultured seed explants of peanut (Arachis hypogaea L.) using thidiazuron. In vitro Cell Dev. Biol. Plant 30:187–191; 1994.

    Google Scholar 

  • Malik, K. A.; Saxena, P. K. Regeneration in Phaseolus vulgaris L. Highfrequency induction of direct shoot formation in intact seedlings by N 6-benzyladenine and thidiazuron. Planta 186:384–389; 1992.

    Article  CAS  Google Scholar 

  • Mok, M. C.; Mok, D. W. S.; Armstrong, D. J.; Shudo, I. O.; Isogai, Y.; Okamoto, T. Cytokinin activity of N-phenyl-N'-1,2,3-thidiazol-5-ylurca (thidiazuron). Phytochemistry 21:1509–1511; 1982.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol. Plant. 94:268–276; 1995.

    Article  CAS  Google Scholar 

  • Murthy, B. N. S.; Murch, S. J.; Saxena, P. K. Thidiazuron: a potent regulator of in vitro plant morphogenesis. In vitro. Cell. Dev. Biol. Plant. 34:267–275; 1998.

    CAS  Google Scholar 

  • Pedersen, M. G.; Andersen, J. M. Callus morphology and endogenous cytokinin in regenerating sugar beet callus cultures. Proceedings of the Beta Eucarpia Meeting Helsingor; 1998

  • Pua, E. C.; Thorpe, T. A. Differential response of non-selected and Na2SO4-selected callus cultures of Beta vulgaris L. to salt stress. J. Plant Physiol. 123:241–248; 1986.

    CAS  Google Scholar 

  • Ritchie, G. A.; Short, K. C.; Davey, M. R. In vitro shoot regeneration from callus, leaf axils and petioles of sugar beet (Beta vulgaris L.). J. Exp. Bot. 402:277–283; 1989.

    Article  Google Scholar 

  • Roussy, I.; Doubois, F.; Sangwan, R. S.; Sangwan-Norreel, B. S. In planta 2,3,5-triiodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants Plant Cell Rep 16:142–146; 1996.

    CAS  Google Scholar 

  • Saunders, J. W.; Acquaah, G.; Renner, K. A.; Doley, W. P. Monogenic dominant sulfonylurea resistance in sugarbeet from somatic cell selection. Crop Sci. 32:1357–1360; 1992.

    Article  CAS  Google Scholar 

  • Saunders, J. W.; Doley, W. P. One step shoot regeneration from callus of whole plant leaf explants of sugarbeet lines and somaclonal variation of in vitro culture behaviour. J. Plant Physiol. 124:473–479; 1986.

    CAS  Google Scholar 

  • Saxena, P. K.; Malik, K. A.; Gill, R. Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut. Planta 187:421–424; 1992.

    Article  CAS  Google Scholar 

  • Tenning, P.; Weich, E. W.; Kjarsgaard, U.-B.; Lelu, M.-A.; Nihlgard, M. Somatic embryogenesis form zygotic embryos of sugarbeet (Beta vulgaris L.). Plant Sci. 81:103–109; 1992.

    Article  Google Scholar 

  • Tetu, T.; Sangwan, R. S.; Sangwan-Narreel, B. S. Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J. Exp. Bot. 38:506–517; 1987.

    Article  CAS  Google Scholar 

  • Thomas, J. C.; Katterman, F. R. Cytokinin activity induced by thidiazuron. Plant Physiol 81:681–683; 1986.

    Article  PubMed  CAS  Google Scholar 

  • Yu, M. H. Growth and reproduction performance of ovule-induced sugarbeet plants. SABRAO J. 24:47–55; 1992.

    Google Scholar 

  • Zhong, Z.; Smith, H. G.; Thomas, T. H. In vitro culture of petioles and intact leaves of sugar beet (Beta vulgaris). Plant Growth Reg. 12:59–66; 1993.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The Norman Borlaug Institute for Plant Science Research, De Montfort University, LE7 9SU, Scraptoft, Leicester, UK

    Chun-Lai Zhang, Dong-Fang Chen, Malcolm C. Elliott & Adrian Slater

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  1. Chun-Lai Zhang
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  2. Dong-Fang Chen
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  3. Malcolm C. Elliott
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  4. Adrian Slater
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Correspondence to Adrian Slater.

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Zhang, CL., Chen, DF., Elliott, M.C. et al. Thidiazuron-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.). In Vitro Cell.Dev.Biol.-Plant 37, 305–310 (2001). https://doi.org/10.1007/s11627-001-0054-6

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  • DOI: https://doi.org/10.1007/s11627-001-0054-6

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