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The anti-aging effects of Ludwigia octovalvis on Drosophila melanogaster and SAMP8 mice

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Abstract

We investigated the anti-aging effects of Ludwigia octovalvis (Jacq.) P. H. Raven (Onagraceae), an extract of which is widely consumed as a healthful drink in a number of countries. Using the fruit fly, Drosophila melanogaster, as a model organism, we demonstrated that L. octovalvis extract (LOE) significantly extended fly lifespan on a high, but not a low, calorie diet, indicating that LOE may regulate lifespan through a dietary restriction (DR)-related pathway. LOE also attenuated age-related cognitive decline in both flies and in the senescence-accelerated-prone 8 (SAMP8) mouse, without causing any discernable negative trade-offs, including water intake, food intake, fecundity, or spontaneous motor activity. LOE contained high levels of polyphenols and flavonoids, which possess strong DPPH radical scavenging activity, and was shown to attenuate paraquat-induced oxidative damage and lethality in flies. Gas chromatography–mass spectrometry (GC-MS) analyses identified 17 known molecules, of which β-sitosterol and squalene were the two most abundant. We further demonstrated that β-sitosterol was capable of extending lifespan, likely through activating AMP-activated protein kinase (AMPK) in the fat body of adult flies. Taken together, our data suggest that LOE is a potent anti-aging intervention with potential for treating age-related disorders.

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Acknowledgments

We would like to thank Chuan-Hao Lai and Hao-Wei Wang (Hsiehyu Biotech Company Ltd.) and Shang-Tse Lee (National Taiwan University) for the technical assistance. We would like to thank Fly Core in Taiwan for the fly stocks and reagents. Pei-Yu Wang is partially supported by the National Science Council (NSC 98-2320-B-004-003-MY2 and NSC 100-2311-B-002-017-MY3), Taiwan.

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Authors and Affiliations

  1. Graduate Institute of Brain and Mind Sciences, College of Medicine, National Taiwan University, No. 1, Sec. 1, Renai Rd., Taipei, 10051, Taiwan

    Wei-Sheng Lin, Jo-Chiao Wang & Pei-Yu Wang

  2. Institute of Neuroscience, National Chengchi University, Taipei, Taiwan

    Jun-Yi Chen

  3. Department of Biotechnology, Mingchuan University, Taoyuan, Taiwan

    Liang-Yu Chen

  4. Hsiehyu Biotech Company Ltd., Taipei, Taiwan

    Che-Hao Lin & Tsung-Ren Hsieh

  5. Department of Food and Nutrition, Providence University, Taichung, Taiwan

    Ming-Fu Wang

  6. Department of Applied Chemistry, National Chinan University, Nantou, Taiwan

    Tsai-Feng Fu

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  1. Wei-Sheng Lin
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Wei-Sheng Lin and Jun-Yi Chen contributed equally to this work.

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Fig. S1

Reduced expression of AMPK-α mRNA caused by expression of the RNAi element. qPCR detection of mRNA expression levels of AMPK-α in 10-day old flies carrying fat body GeneSwitch driver S106 and UAS-AMPK.RNAi, on food containing 0 μg/ml (control) and 86 μg/ml RU486(RU). Data are presented as mean ± SEM. Experiments were done in triplicates and each sample contained more than 30 flies. *, P < 0.05, compared to control group using Student's t test. (JPEG 1898 kb)

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Lin, WS., Chen, JY., Wang, JC. et al. The anti-aging effects of Ludwigia octovalvis on Drosophila melanogaster and SAMP8 mice. AGE 36, 689–703 (2014). https://doi.org/10.1007/s11357-013-9606-z

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