Skip to main content
SpringerLink
Log in

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

To induce variation through chromosome doubling in Gerbera jamesonii Bolus cv. Sciella, two-week-old in vitro grown shoots were treated with various concentrations of colchicine (0.01, 0.05, 0.10, 0.50 or 1% w/v) for 2, 4 or 8 h. Treated shoots were then cultured on Murashige and Skoog (MS) medium supplemented with 8.8 μM 6-benzyladenine (BA) and 155 μM adenine sulphate (ADS), and subsequently transferred to fresh MS medium containing 2.85 μM indole-3 acetic acid (IAA) for rooting. When shoots were treated with 0.1% colchicine for 8 h, 64% of recovered plantlets were tetraploid. Ploidy of plantlets was confirmed by flow cytometry, stomatal analysis, and morphological characters. Tetraploid plantlets displayed slower proliferation along with higher vigor and thickened broad leaves. Moreover, tetraploid plants developed larger flowers, longer stalks, and have improved vase-life, all contributing to higher ornamental value of gerbera.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

ADS:

Adenine sulphate

BA:

6-Benzyladenine

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog (1962)

PGR:

Plant growth regulator

References

  • Boaventura YMS, Medina DM, Vieira MJFR, Arruda HV (1981) Número de cloroplastos e nĭvel de ploidia em espécies de Coffea L. Rev Bras Bot 4:15–21

    Google Scholar 

  • Carvalho R, Guerra M, Carvalho PCL (1999) Occurrence of spontaneous triploids in Manihot esculenta Cruntz. Cytologia 64:137–140

    Google Scholar 

  • Chavadej S, Becker H (1984) Influence of colchicine treatment on chromosome number and growth rate of tissue cultures of Valeriana Wallichii DC. Plant Cell Tiss Organ Cult 3:265–272

    Article  CAS  Google Scholar 

  • Chen WH, Tang CY, Kao YL (2009) Ploidy doubling by in vitro culture of excised protocorms or protocorms-like bodies in Phalaenopsis species. Plant Cell Tiss Organ Cult 98:229–238

    Article  CAS  Google Scholar 

  • Clarke J (1960) Preparation of leaf epidermis for tropographic study. Stain Technol 35:35–39

    PubMed  CAS  Google Scholar 

  • Cramer CS (1991) Hybridization between diploid and tetraploid Pelargonium × hortorum Bailey. UnderGraduate Honourse Thesis, Pa. State Univ., University Park

  • De Oliveira VM, Forni-Martins ER, Magalhães PM, Alves MN (2004) Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) (Eupatorieae, Asteraceae). Genet Mol Biol 27:215–222

    Article  Google Scholar 

  • Devadasan K (2008) Diversification and value addition are the key words. The Hindu Survey Ind Agric :97–101

  • Dhooghe E, Van Laere K, Eeckhaut T, Leus L, Van Huylenbroeck J (2011) Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tiss Organ Cult 104:329–341

    Article  Google Scholar 

  • Dickson EE, Arumuganathan K, Koresovich S, Doyle JJ (1992) Nuclear DNA content variation within the Rosaceae. Am J Bot 79:1081–1086

    Article  Google Scholar 

  • Dolezel J, Greilhuber J, Suda J (2007) Flow cytometry with plants: an overview. In: Dolezel J, Greilhuber J, Suda J (eds) Flow cytometry with plant cells. Wiley, Weinheim, pp 41–65

    Chapter  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple F test. Biometrics 11:1–42

    Article  Google Scholar 

  • Evans AM (1955) The production and identification of polyploids in red clover, white clover and Lucerne. New Phytol 514:149–162

    Article  Google Scholar 

  • Forni-Martins ER, Cruz ND (1985) Dados preliminaries sobre indução de poliploidia em Jatropha curcas (Euphorbiaceae). Congresso da Sociedade Botànica de São Paulo. V. Botucatu. Resumos, p 167

  • Galbraith DW, Harkns KR, Maddox JM (1983) Rapid flowcytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051

    Article  PubMed  CAS  Google Scholar 

  • Gantait S (2009) Accelerated cloning in vitro and induction of variation in six plants having medicinal, aromatic or aesthetic importance. PhD Thesis, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India

  • Gu XF, Yang AF, Meng H, Zhang JR (2005) In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill cv. Zhanhua. Plant Cell Rep 24:671–676

    Article  PubMed  CAS  Google Scholar 

  • Han L, Yan B, Zhang T, Jiang Y, Zhang H, Yu L, Li S (2009) Preliminary studies on polyploidy mutation of cut flower Gerbera jamesonii Bolus. Acta Hortic Sinica 36:605–610

    Google Scholar 

  • Honkanen J, Aapola A, Seppänen P, Törmälä T, Oy K, de Wit JC, Esendam HF, Stravers LJM, Terra Nigra BV (1991) Production of double haploid gerbera clones. Acta Hortic 300:341–346

    Google Scholar 

  • Kang XY (2003) Advances in researches on polyploid breeding of forest trees. J Beijing For Univ 25:70–74

    Google Scholar 

  • Kanwar JK, Kumar S (2008) In vitro propagation of Gerbera—a review. Hort Sci 35:35–44

    CAS  Google Scholar 

  • Leblanc O, Hernández M, Bello S, Garcia V, Bertaud J, Savidan Y (1995) Chromosome doubling in Tripsacum: the production of artificial, sexual tetraploid plants. Plant Breed 114:226–230

    Article  Google Scholar 

  • Liu G, Li Z, Bao M (2007) Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology. Euphytica 157:145–154

    Article  Google Scholar 

  • Lyrene PM, Perry JL (1982) Production and selection of blueberry polyploids in vitro. J Hered 73:377–378

    Google Scholar 

  • Miyoshi K, Asakura N (1996) Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Rep 16:1–5

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nguyen T, Phuong T, Kenji U, Ikuo M, Yukio O, Hiroshi O (2003) Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell Tiss Organ Cult 72:19–25

    Article  Google Scholar 

  • Ochatt SJ, Patat-Ochatt EM, Moessner A (2011) Ploidy level determination within the context of in vitro breeding. Plant Cell Tiss Organ Cult 104:329–341

    Article  Google Scholar 

  • Orlikowska T, Nowak E, Marasek A, Kucharska D (1999) Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petiole. Plant Cell Tiss Organ Cult 59:95–102

    Article  CAS  Google Scholar 

  • Parthasarathy VA, Nagaraju V (1999) In vitro propagation in Gerbera jemesonii Bolus. Ind J Hortic 56:82–85

    Google Scholar 

  • Pierik RLM, Steegmans HHM, Marelis JJ (1973) Gerbera plantlets from in vitro cultivated capitulum explants. Sci Hortic 1:117–119

    Article  Google Scholar 

  • Pinheiro AA, Pozzobon MT, do Valle CB, Penteado MIO, Carneiro VTC (2000) Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep 19:274–278

    Article  CAS  Google Scholar 

  • Predieri S (2001) Mutation induction and tissue culture in improving fruits. Plant Cell Tiss Organ Cult 64:185–210

    Article  CAS  Google Scholar 

  • Rose JB, Kubba J, Tobutt KR (2001) Induction of tetraploids for breeding hardy ornamentals. Acta Hortic 560:109–112

    CAS  Google Scholar 

  • Roy AT, Leggett G, Koutoulis A (2001) In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Rep 20:489–495

    Article  CAS  Google Scholar 

  • Shao JZ, Chen CL, Deng XX (2003) In vitro induction of tetraploid in pomegranate (Punica granatum). Plant Cell Tiss Organ Cult 75:241–246

    Article  CAS  Google Scholar 

  • Shapiro HM (2003) Practical flow cytometry, 4th edn. John Wiley & Sons, Hoboken, N.J

    Book  Google Scholar 

  • Song P, Kang W, Peffley EB (1997) Chromosome doubling of Allium fistulosum 9 A. cepa interspecific F1 hybrids through colchicine treatment of regenerating callus. Euphytica 93:257–262

    Article  Google Scholar 

  • Speckman GJ, Post J, Dijkstra M (1965) The length of stomata as an indicator for polyploidy in rye-grass. Euphytica 14:225–230

    Article  Google Scholar 

  • Stebbins GL (1984) Polyploidy and the distributed of arctic-alpine flora: a new evidence and new approaches. Bot Helv 94:1–13

    Google Scholar 

  • Tang ZQ, Chen DL, Song ZJ, He YC, Cai DT (2010) In vitro induction and identification of tetraploid plants of Paulownia tomentosa. Plant Cell Tiss Organ Cult 102:213–220

    Article  Google Scholar 

  • Tosca A, Pandolfi R, Citterio S, Fasoli A, Sgorbati S (1995) Determination by flow cytometry of the chromosome doubling capacity of colchicine and oryzalin in gynogenetic haploids of Gerbera. Plant Cell Rep 14:455–458

    Article  CAS  Google Scholar 

  • Uhlik J (1981) Compendium postgradulans stadium for Genetics and Breeding. Prague V.S.Z. pp 105–195

  • Viehmannova I, Fernandez E, Bechyne M, Vyvadilova M, Greplova M (2009) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius). Plant Cell Tiss Organ Cult 95:21–25

    Article  Google Scholar 

  • Wang L, Zheng SX, Gu ZJ (2002) In vitro culture of tetraploids of Aloe vera L. Acta Hortic Sinica 29:176–178

    Article  Google Scholar 

  • Wright JW (1976) Introduction of forest genetics. Academic Press, New York

    Google Scholar 

  • Yang X, Ye CY, Cheng ZM, Tschaplinski TJ, Wullschleger SD, Yin W, Xia X, Tuskan GA (2011) Genomic aspects of research involving polyploid plants. Plant Cell Tiss Organ Cult 104:387–397

    Article  Google Scholar 

  • Zhang J, Zhang M, Deng X (2007) Obtaining autotetraplids in vitro at a high frequency in Citrus sinensis. Plant Cell Tiss Organ Cult 89:211–216

    Article  Google Scholar 

  • Zhang QY, Luo FX, Liu L, Guo FC (2010) In vitro induction of tetraploids in crape myrtle (Lagerstoemia indica L.). Plant Cell Tiss Organ Cult 101:41–47

    Article  CAS  Google Scholar 

  • Zhu ZT, Kang XY, Zhang ZY (1998) Studies on selection of natural triploids of Populus tomentosa. Sci Silvae Sinicae 34:22–31

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Instrumentation and Environmental Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, WB, 741252, India

    Saikat Gantait & Nirmal Mandal

  2. Department of Genetics, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, WB, 741252, India

    Somnath Bhattacharyya & Prakash Kanti Das

Authors
  1. Saikat Gantait
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nirmal Mandal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Somnath Bhattacharyya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Prakash Kanti Das
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Saikat Gantait.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gantait, S., Mandal, N., Bhattacharyya, S. et al. Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell Tiss Organ Cult 106, 485–493 (2011). https://doi.org/10.1007/s11240-011-9947-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-011-9947-1

Keywords

Search

Navigation