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Primary and repetitive secondary somatic embryogenesis in Rosa hybrida ‘Samantha’

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Abstract

This study describes a plant regeneration system for Rosa hybrida ‘Samantha’, a mainstream commercial cultivar, via direct somatic embryogenesis. Somatic embryogenesis was induced from in vitro-derived leaf explants, achieving a frequency of 7.5% following 8 weeks of culture on a Murashige and Skoog (MS) medium supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 30 g/L glucose. We evaluated the effects of various types and concentrations of carbohydrates and plant growth regulators (PGRs) on the proliferation and germination of secondary somatic embryos. MS medium containing 1.0 mg/L 2,4-D, 0.05 mg/L 6-benzyladenine (BA) and 60 g/L glucose was found to be the most effective in promoting the proliferation of somatic embryos. The highest germination rate (56.2%) of somatic embryos was observed on MS medium containing 1.0 mg/L Thidiazuron (TDZ) and 0.5 mg/L BA. Whole plantlets were obtained by culturing germinated shoots on rooting medium comprising 1/2-strength MS salts supplemented with 0.05 mg/L α-Naphthalene acetic acid (NAA). Plantlets grew vigorously with normal vegetative and flowering characteristics.

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Abbreviations

MS:

Murashige and Skoog (1962)

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

TDZ:

Thidiazuron

BA:

6-Benzyladenine

NAA:

α-Naphthalene acetic acid

GA3 :

Gibberellic acid

l-p:

l-proline

PGR:

Plant growth regulator

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Acknowledgments

This research was supported by the National Key Forestry Technology R&D Program (2006BAD01A1805) and the Special Fund for Agro-scientific Research in the Public Interest (200903020). We thank all of the colleagues in our laboratory for constructive discussion and technical support. We are also grateful to Dr. Alex C. McCormac for critical editing of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Ying Bao, Guofeng Liu, Xueping Shi, Wen Xing, Guogui Ning, Juan Liu & Manzhu Bao

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  1. Ying Bao
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  2. Guofeng Liu
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  3. Xueping Shi
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  4. Wen Xing
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  5. Guogui Ning
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  6. Juan Liu
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  7. Manzhu Bao
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Correspondence to Manzhu Bao.

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Bao, Y., Liu, G., Shi, X. et al. Primary and repetitive secondary somatic embryogenesis in Rosa hybrida ‘Samantha’. Plant Cell Tiss Organ Cult 109, 411–418 (2012). https://doi.org/10.1007/s11240-011-0105-6

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  • DOI: https://doi.org/10.1007/s11240-011-0105-6

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