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Induction of metal binding compounds and antioxidative defence in callus cultures of two black poplar (P. nigra) clones with different tolerance to cadmium

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Abstract

Callus cultures of two parental clones of Populus nigra L., Poli and 58-861, originating from contrasting environments, were exposed to different cadmium concentrations (0, 150 and 250 μM CdSO4). Clones showed different growth responses to cadmium, evaluated by the tolerance index (Ti), with Poli being more tolerant to the metal at both concentrations. The cadmium concentration at the end of the treatment was very similar between clones at 150 μM CdSO4, while a higher value in 58-861 compared to Poli was detected at 250 μM CdSO4. The bioconcentration factor evidenced the lowest value in Poli at 250 μM CdSO4. Unlike 58-861, cadmium provoked a strong induction of thiols and phytochelatins in clone Poli. In both clones, organic acid concentration differed notably in untreated calli and cadmium treatment induced a general lowering of these compounds. A notably higher antioxidant enzyme activity (ascorbate peroxidase, APX; catalase, CAT; guaiacol peroxidase, GPX) was measured in control calli of clone Poli compared to 58-861. Cadmium induced a remarkable enhancement of APX and CAT, but not GPX, activity at 150 μM CdSO4 in Poli. Conversely, in 58-861 at 150 μM CdSO4, and in both clones at 250 μM CdSO4, a decrease in the antioxidant activity occurred. This investigation provided evidence that these two contrasting genotypes of P. nigra are characterised by a different response to cadmium in callus cultures. In particular, in Poli, the higher tolerance to cadmium is associated with a higher activity of antioxidative enzymes and the ability to strongly increase thiol and PC concentration in response to metal exposure.

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Abbreviations

APX:

Ascorbate peroxidase

BCF:

Bioconcentration factor

CAT:

Catalase

Cys:

Cysteine

γ-EC:

γ-Glutamylcysteine

GPX:

Guaiacol peroxidase

GSH:

Glutathione

MS:

Murashige and Skoog

OAs:

Organic acids

PCs:

Phytochelatins

Ti:

Tolerance index

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Authors and Affiliations

  1. Institute of Agro-environmental and Forest Biology, CNR, National Research Council of Italy, Via Salaria Km. 29,300, 00015, Monterotondo Scalo, Rome, Italy

    Valentina Iori, Fabrizio Pietrini, Angelo Massacci & Massimo Zacchini

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  1. Valentina Iori
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  2. Fabrizio Pietrini
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  3. Angelo Massacci
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  4. Massimo Zacchini
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Correspondence to Massimo Zacchini.

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Iori, V., Pietrini, F., Massacci, A. et al. Induction of metal binding compounds and antioxidative defence in callus cultures of two black poplar (P. nigra) clones with different tolerance to cadmium. Plant Cell Tiss Organ Cult 108, 17–26 (2012). https://doi.org/10.1007/s11240-011-0006-8

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