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Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

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Abstract

Transgenic plants of rose-scented geranium (Pelargonium graveolens cv. Hemanti) have been produced from Agrobacterium rhizogenes (strains A4 and LBA9402) mediated hairy root cultures. Amongst the explants tested, leaves were most responsive followed by the petioles and internodal segments, respectively. The A4 strain performed better for all the three explants both in terms of frequency of response and time requirement for hairy root induction. Transgenic shoots could be obtained by spontaneous regeneration without intervening callus phase amongst 16% and 12% root lines of A4 and LBA 9402 origin, respectively, or they were induced in 29% and 22% hairy root lines of A4 and LBA9402 origin, respectively, with different hormonal supplementation. These transgenic plants showed 30% survival as against 90% of their control under the confined environment of glasshouse. The transgenic plants were of similar morphotype having increased branching, higher number of leaves with increased dentations, short and round stature, highly branched root system and absence of leaf wrinkling. These transgenic plants showed opine positive results even after 5 months of their transfer to the glasshouse. The essential oil compositions of 81% of these transgenics were qualitatively similar to that of the wild type parent. However, two transgenic plants (LZ-3 and 14TG) showed increase in concentrations of geraniol and geranyl esters signifying improved oil quality with respect to the citronellol:geraniol ratio. These two oils having better olfactory value represent an improvement over that of the wild type parent from the commercial point of view.

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Abbreviations

MS:

Murashige and Skoog’s medium

IBA:

Indole butyric acid

BAP:

6-Benzylaminopurine

NAA:

Naphthaleneacetic acid

GC:

Gas chromatography

GC/MS:

Gas chromatography mass spectroscopy

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Acknowledgements

The authors are thankful to the Department of Biotechnology, Government of India for the financial support and the Regional Sophistication Instrumentation Facility, CDRI, Lucknow for GC-MS of the oils. Thanks are also due to S. Sharma and S.A. Hasan for their help in manuscript preparation and A.P. Dhiman for photography.

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Authors and Affiliations

  1. Department of Botany, Lucknow University, Lucknow, 226001, India

    Gauri Saxena

  2. Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, 226015, India

    Suchitra Banerjee

  3. CIMAP Field Station, Puraura, India

    Laiq-ur-Rahman

  4. National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India

    Praveen Chandra Verma

  5. CIMAP Field Station, Bangalore, India

    G. R. Mallavarapu

  6. National Centre for Plant Genome Research, JNU Campus, P.O. Box 10531, New Delhi, 110067, India

    Sushil Kumar

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  1. Gauri Saxena
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  2. Suchitra Banerjee
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  3. Laiq-ur-Rahman
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  4. Praveen Chandra Verma
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  5. G. R. Mallavarapu
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  6. Sushil Kumar
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Correspondence to Gauri Saxena.

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Saxena, G., Banerjee, S., Laiq-ur-Rahman et al. Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality. Plant Cell Tiss Organ Cult 90, 215–223 (2007). https://doi.org/10.1007/s11240-007-9261-0

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  • DOI: https://doi.org/10.1007/s11240-007-9261-0

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