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Artificial Niches for Stromal Stem Cells as a Potential Instrument for the Design of the Surface of Biomimetic Osteogenic Materials

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Russian Physics Journal Aims and scope

A relationship between the topography of rough calcium phosphate surfaces having osteogenic niche-reliefs and the electrostatic potential of these surfaces as a possible instrument to control stromal stem cells has been investigated. The in vitro culture of human lung prenatal stromal cells on nanostructured/ultrafine-grained VT1.0 titanium alloy plates with bilateral rough calcium phosphate (CaP) microarc coating was used. It was established that the amplitude of the electret CaP surface potential linearly increased with increasing area of valleys (sockets), and the negative charge is formed on the socket surface. The area of alkaline phosphatase staining (the marker of osteoblast maturation and differentiation) of adherent CD34– CD44+ cells increases linearly with increasing area of artificial microterritory (socket) of the CaP surface occupied with each cell. The negative electret potential in valleys (sockets) of microarc CaP coatings can be the physical mechanism mediating the influence of the surface topography on osteogenic maturation and differentiation of cells in vitro. This mechanism can be called “niche-potential” and can be used as an instrument for biomimetic modification of smooth CaP surfaces to strengthen their integration with the bone tissue.

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Authors and Affiliations

  1. Siberian State Medical University, Tomsk, Russia

    I. A. Khlusov & M. Yu. Khlusova

  2. National Research Tomsk Polytechnic University, Tomsk, Russia

    I. A. Khlusov & Yu. P. Sharkeev

  3. Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, Tomsk, Russia

    V. F. Pichugin, Yu. P. Sharkeev & E. V. Legostaeva

Authors
  1. I. A. Khlusov
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  2. M. Yu. Khlusova
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  3. V. F. Pichugin
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  4. Yu. P. Sharkeev
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  5. E. V. Legostaeva
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Correspondence to I. A. Khlusov.

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Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 10, pp. 92–97, October, 2013.

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Khlusov, I.A., Khlusova, M.Y., Pichugin, V.F. et al. Artificial Niches for Stromal Stem Cells as a Potential Instrument for the Design of the Surface of Biomimetic Osteogenic Materials. Russ Phys J 56, 1206–1211 (2014). https://doi.org/10.1007/s11182-014-0163-4

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  • DOI: https://doi.org/10.1007/s11182-014-0163-4

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