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Activation of the oxidative burst by yeast elicitor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis

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Abstract

In Catharanthus roseus cell suspensions, expression of several terpenoid indole alkaloid (TIA) biosynthetic genes, including those encoding strictosidine synthase and tryptophan decarboxylase, is coordinately induced by fungal elicitors such as yeast extract (YE). This induction is mediated by several signaling steps including the biosynthesis of jasmonic acid, and the activation of the jasmonic acid-responsive ORCA transcription factors. We investigated a possible role of reactive oxygen species (ROS) as a second messenger in this system. YE was shown to activate the production of ROS, which was dependent on protein phosphorylation and calcium in.ux. However, ROS generation was neither necessary for the induction of genes involved in TIA biosynthesis by YE nor by itself su.cient to induce these genes. Therefore, we conclude that activation of the oxidative burst by YE occurs independently of the activation of genes involved in TIA biosynthesis.

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Abbreviations

DCFH-DA:

dichlorodihydrofluorescein diacetate

DPI:

diphenylene iodonium

JA:

jasmonic acid

MeJA:

methyljasmonate

NAC:

N-acetyl-L-cysteine

ORCA:

octadecanoid-responsive Catharanthus AP2-domain

ROS:

reactive oxygen species

STR:

strictosidine synthase

TDC:

tryptophan decarboxylase

TIA:

terpenoid indole alkaloid

YE:

yeast elicitor

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Authors and Affiliations

  1. Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, AL Leiden, The Netherlands

    Bea Pauw, Jan W. Kijne & Johan Memelink

  2. Department of Applied Plant Sciences, The Netherlands Organisation for Applied Scientific Research, Zernikedreef 9, 2333, CK Leiden, The Netherlands

    Bert van Duijn

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  1. Bea Pauw
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  3. Jan W. Kijne
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Correspondence to Johan Memelink.

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Pauw, B., van Duijn, B., Kijne, J.W. et al. Activation of the oxidative burst by yeast elicitor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis. Plant Mol Biol 55, 797–805 (2004). https://doi.org/10.1007/s11103-005-1968-x

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