Abstract
High glycemic index diet can induce multiple diseases. Many research indicated that oxidative stress played important role in many pathological conditions. However, the impact of gene expression and dietary habit on oxidation process are still less clear. We used high-glucose diet to feed C57BL/6J mice for 4 weeks, measured the redox status, physiological and biochemical changes related to diabetes and consequence of metabolic syndrome (nonalcoholic fatty liver, cardiovascular disease), and detected the expressions of 14,446 genes in liver of C57BL/6J mice with DNA microarray. The results showed high-glucose diet induced elevated fatty acid accumulation in liver, insulin resistance index and higher weight in C57BL/6J mice, which indicated high-glucose diet caused to the initiation and development of diabetes and consequence of metabolic syndrome. The results also showed high-glucose diet induced oxidative stress in liver of C57BL/6J mice, which might the cause of initiation and development of diabetes and consequence of metabolic syndrome. Microarray analysis found expressions of genes related to thiol redox, fatty acid oxidation in peroxisome and cytochrome P450 were significantly changed, indicating system in which non-enzyme antioxidant capacity was impaired and sources from which reactive oxygen species (ROS) generated, which revealed the molecular mechanism of oxidative stress induced by high-glucose diet. We validated our microarray findings by conducting real-time RT–PCR assays on selected genes.
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References
WHO (2006) World health statistics, 2006. World Health Organization, Geneva, Switzerland
WHO (2000) Diet, nutrition, the prevention of chronic disease. World Health Organ Tech Rep Ser 916:1–149
Barclay AW, Petocz P, Mcmillan-Price J, Flood VM, Prvan T, Mitchell P, Brand-Miller JC (2008) Glycemic index, glycemic load, and chronic disease risk: a meta-analysis of observational studies. Am J Clin Nutr 87:627–637
Le KA, Bortolotti M (2008) Role of dietary carbohydrates and macronutrients in the pathogenesis of nonalcoholic fatty liver disease. Curr Opin Clin Nutr Metab Care 11:477–482
Mulholland HG, Murray LJ, Cardwell CR, Cantwell MM (2008) Dietary glycemic index, glycemic load and endometrial and ovarian cancer risk: a systematic review and meta-analysis. Br J Cancer 99:434–441
Brwonlee M (2005) The pathobiology of diabetic complications: a unifying mechanism. Diabetes 54:1615–1625
Ceriello A, Quagliaro L, Piconi L, Assaloni R, Da Ros R, Maier A, Esposito K, Giugliano D (2004) Effect of postprandial hypertriglyceridemia and hyperglycemia on circulating adhesion molecules and oxidative stress generation and the possible role of simvastatin treatment. Diabetes 53:701–710
Mohanty P, Hamouda W, Garg R, Aljada A, Ghanim H, Dandona P (2000) Glucose challenge stimulates reactive oxygen species (ROS) generation by leucocytes. J Clin Endocrinol Metab 85:2970–2973
Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291–295
Eriksson JW (2007) Metabolic stress in insulin’s target cells leads to ROS accumulation: a hypothetical common pathway causing insulin resistance. FEBS Lett 581:3734–3742
Holvoet P, Lee DH, Steffes M, Gross M, Jacobs DR (2008) Association between circulating oxidized low-density lipoprotein and incidence of the metabolic syndrome. JAMA 299:2287–2293
Duncan ER, Walker SJ, Ezzat VA, Wheatcroft SB, Li JM, Shah AM, Kearney MT (2007) Accelerated endothelial dysfunction in mild prediabetic insulin resistance: the early role of reactive oxygen species. Am J Physiol Endocrinol Metab 293:E1311–E1319
Du D, Shi YH, Le GW (2009) Microarray analysis of high-glucose diet-induced changes in mRNA expression in jejunums of C57BL/6J mice reveals impairment in digestion, absorption. Mol Biol Rep. doi:10.1007/s11033-009-9622-3
Duseja A, Thumburu KK, Das A, Dhiman PK, Chawla YK, Bhadada S, Bhansali A (2007) Insulin tolerance test is comparable to homeostasis model assessment for insulin resistance in patients with nonalcoholic fatty liver disease. Indian J Gastroenterol 26:170–173
Hafner SM, Kennedy E, Gonzalez C, Stern MP, Miettinen H (1996) A prospective analysis of the HOMA model. The Mexico City Diabetes Study. Diabetes Care 19:1138–1141
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Vaca CE, Wilhelm J, Harms-Rihsdahl M (1988) Interaction of lipid peroxidation product with DNA: a review. Mutat Res Rev Genet Toxicol 195:137–149
Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358
Raza H, Robin MA, Fang JK, Avadhani NG (2002) Multiple isoforms of mitochondrial glutathione S-transferases and their differential induction under oxidative stress. Biochem J 366:45–55
Tang JJ, Wang MW, Jia EZ, Yan JJ, Wang QM, Zhu J, Yang ZJ, Lu X, Wang LS (2010) The common variant in the GSTM1 and GSTT1 genes is related to markers of oxidative stress and inflammation in patients with coronary artery disease: a case-only study. Mol Biol Rep 37:405–410
Janiszewski M, Lopes LR, Carmo AO, Pedro MA, Brandes RP, Santos CX, Laurindo FR (2005) Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells. J Biol Chem 280:40813–40819
Maghdooni Bagheri P, Govaerts I, De Ley M (2010) Role of metallothionein in differentiation of leukemia cells. Mol Biol Rep. 2010 Feb 3. [Epub ahead of print] PMID: 20127519
Thornalley PJ, Vasak M (1985) Possible role for metallothionein in protection against radiation-induced oxidative stress: kinetics and mechanism of its reaction with superoxide and hydroxyl radicals. Biochim Biophys Acta 827:36–44
Bremner I (1987) Nutritional and physiologic significance of metallothionein. Exp Suppl 52:81–107
Suemori S, Sbimazawa M, Kawase K, Satob M, Nagase H, Yamamoto T, Hara H (2006) Metallothionein, an endogenous antioxidant, protects against retinal neuron damage in mice. Invest Ophthalmol Vis Sci 47:3975–3982
Ohgami RS, Campagna DR, McDonald A, Fleming MD (2006) The Steap proteins are metalloreductases. Blood 108:1388–1394
Johnson EF, Palmer GN, Griffin KJ, Hsu MH (1996) Role of the peroxisome proliferator-activated receptor in cytochrome P450 4A gene regulation. FASEB J 10:1241–1248
Mannaerts GP, Van Veldhoven PP, Gastells M (2000) Peroxisome lipid degradation via β- and α-oxidation in mammals. Cell Biochem Biophys 32:73–87
Reddy JK, Hshimoto T (2001) Peroxisomal β-oxidation and peroxisome proliferator-activated receptor: an adaptive metabolic system. Annu Rev Nutr 21:193–230
Li X, Baumqart E, Donq GX, Morrell JC, Jimenez-Sanchez G, Valle D, Smith KD, Gould SJ (2002) PEX11alpha is required for peroxisome proliferation in response to 4-phenylbutyrate but is dispensable for peroxisome proliferator-activated receptor alpha-mediated peroxisome proliferation. Mol Cell Biol 22:8226–8240
Lieber CS (1997) Cytochrome P-4502E1: its physiological and pathological role. Physiol Rev 77:517–544
Ejstrom G, Ingelman-Sundberg M (1989) Rat liver microsomal NADPH-supported oxidase activity and lipid peroxidation dependent on ethanol-inducible cytochrome P-450 (P-450IIE1). Biochem Pharmacol 38:1313–1319
Leclercq IA, Farrell GC, Field J, Bell DR, Gonzalez FJ, Robertson GR (2000) CYP2E1 and CYP4A as microsomal catalysts of lipid peroxides in murine nonalcoholic steatohepatitis. J Clin Invest 105:1067–1075
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This work was supported by National Natural Science Foundation of China (No. 30571347).
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Du, D., Shi, YH. & Le, GW. Oxidative stress induced by high-glucose diet in liver of C57BL/6J mice and its underlying mechanism. Mol Biol Rep 37, 3833–3839 (2010). https://doi.org/10.1007/s11033-010-0039-9
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DOI: https://doi.org/10.1007/s11033-010-0039-9