Abstract
In this paper, the DNA fragment of trypsin genes from eight crustaceans were cloned and sequenced. The amino acid composition of the 24 deduced and 42 selected trypsin sequences were compared. Low arginine, methionine, and proline content and high aspartic acid, glutamic acid, and isoleucine content attributed to the distinct catalytic efficiency of crustacean trypsins.
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References
Aittaleb M, Hubner R, Lamotte-Brasseur J, Gerday C (1997) Protein Eng 10:475–477
Anheller JE, Hellgren L, Karlstam B, Vincent J (1989) Arch Dermatol Res 281:105–110
Arpigny JL, Feller G, Davail S, Narinx E, Zekhnini Z, Gerday C (1994) In: Gilles R (ed) Advances in comparative and environmental physiology, vol 20. Springer, Berlin, Heidelberg, pp 269–295
Asgeirsson B, Fox JW, Bjarnason JB (1989) Eur J Biochem 180:85–94
Barrick D, Hughson FM, Baldwin RL (1994) J Mol Biol 237:588–601
Berglund GI, Willassen NP, Hordvik A, Smalås AO (1995) Acta Crystallogr D 51:925–937
Britton KL, Baker PJ, Borges KM, Engel PC, Pasquo A, Rice DW, Robb FT, Scandurra R, Stillman TJ, Yip KS (1995) Eur J Biochem 229:688–695
Bustos RO, Romo CR, Healy MG (1999) Process Biochem 35:327–333
Cohen T, Gertler A, Birk Y (1981) Comp Biochem Physiol B 69:639–646
Dittrich BU (1992) Polar Biol 12:269–274
Fehlhammer H, Bode W (1975) J Mol Biol 98:683–692
Feller G, Payan F, Qian M, Haser R, Gerday C (1994) Eur J Biochem 222:441–447
Feller G, Narinx E, Arpigny JL, Aittaleb M, Baise E, Genicot S, Gerday C (1996) FEMS Microbiol Rev 18:189–202
Gassner NC, Baase WA, Matthews BW (1996) Proc Natl Acad Sci USA 93:12155–12158
Genicot S, Feller G, Gerday C (1988) Comp Biochem Physiol B 90(3):601–609
Grant GA, Henderson KO, Eisen AZ, Bradshaw RA (1980) Biochemistry 19:4653–4659
Gudmundsdóttir A, Óskarsson S, Eakin AE, Craik CS, Bjarnason JB (1994) Biochim Biophys Acta 1219:211–214
Gudmundsdóttir E, Spilliaert R, Yang Q, Craik CS, Bjarnason JB, Gudmundsdóttir A (1996) Comp Biochem Physiol B 113:795–801
Haney P, Konisky J, Koretke KK, Luthey-Schulten Z, Wolynes PG (1997) Proteins 28:117–130
Helland R, Leiros I, Willassen NP, Berglund GI, Smalås AO (1998) Eur JBiochem 256:317–324
Jaenicke R (1990) Philos Trans R Soc Lond B 326:535–553
Johnston D, Hermans JM, Yellowlees D (1995) Arch Biochem Biophys 324(1):35–40
Kabsch W, Sander C (1983) Biopolymers 22:2577–2637
Karlsen S, Hough E, Olsen RL (1998) Acta Crystallogr D 54:32–46
Kurtovic I, Marshall SN, Simpson BK (2006) Comp Biochem Physiol B 143:432–440
Leiros H-KS, Willassen NP, Smalås AO (1999) Extremophiles 3:205–219
Leth-Larsen R, Ásgeirsson B, Thorolfsson M, Norregaard-Madsen M, Hojrup P (1996) Biochim Biophys Acta 1297:49–56
Lottenberg R, Jackson CM (1983) Biochim Biophys Acta 742:558–564
Lu PJ, Liu HC, Tsai IH (1990) Biol Chem 371:851–859
Malakauskas SM, Mayo SL (1998) Nat Struct Biol 5:470–475
Martínez A, Olsen RL, Serra JL (1988) Comp Biochem Physiol B 91:677–684
Mekkes J, Le Poole I, Das Pranab K, Bos J, Westerhof W (1998) Wound Repair Regen 6(1):50–57
Mrabet NT, Van Den Broeck A, Van Den Brande I, Stanssens P, Laroche Y, Lambeir AM, Matthijssens G, Jenkins J, Chiadmi M, van Tilbeurgh H, Rey F, Janini J, Quax WJ, Lasters I, de Maeyer M, Wodak SJ (1992) Biochemistry 31:2239–2253
Osnes KK, Mohr V (1985a) Comp Biochem Physiol B 82:559–606
Osnes KK, Mohr V (1985b) Comp Biochem Physiol B 82:607–619
Outzen H, Berglund GI, Smalds AO, Willassen NP (1996) Comp Biochem Physiol B 115(1):33–45
Roach JC, Wang K, Gan L, Hood L (1997) J Mol Evol 45:640–652
Rypniewski WR, Perrakis A, Vorgias CE, Wilson KS (1994) Protein Eng 7:57–64
Sainz JC, García-Carreňo FL, Hernández-Cortés P (2004) Comp Biochem Physiol B 138:155–162
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New York
Simpson BK, Haard NF (1984) Can JBiochem Cell Biol 62:894–900
Suzuki T, Shibata N (1990) Food Rev Int 6:119–147
Tanji M, Yakabe E, Kageyama T, Takahashi K (1996) J Biochem (Tokyo) 120:647–656
Tanner JJ, Hecht RM, Krause KL (1996) Biochemistry 35:2597–2609
Uchida N, Tsukayama K, Nishide E (1984) Bull Jpn Soc Sci Fish 50:313–321
Walsh KA (1970) Methods Enzymol 19:41–63
Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V (1995) Structure (Lond) 3:1147–1158
Yoshinaka R, Suzuki T, Sato M, Ikeda S (1983) Bull Jpn Soc Fish 49:207–212
Acknowledgments
This work was supported by a grant from the National High-Technology Research and Development Program (863 program) of China (No. 2001AA625020) and Department of Science and Technology of Shandong Province.
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Wu, Z., Jiang, G., Wang, N. et al. Relating Trypsin Enzymatic Properties with Amino Acid Composition. Int J Pept Res Ther 14, 81–87 (2008). https://doi.org/10.1007/s10989-007-9108-x
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DOI: https://doi.org/10.1007/s10989-007-9108-x