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The isolation and characterization of putative mesenchymal stem cells from the spiny mouse

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Abstract

The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSCs may be isolated from different species the aim of this study was to isolate and characterize putative bone marrow derived MSCs from the spiny mouse, Acomys cahirinus. MSCs were isolated from the spiny mouse in a traditional manner, and based on plastic adherence, morphology, colony forming unit-fibroblast assays and functional assessment (adipogenic, osteogenic and chondrogenic differentiation potential) a population of putative mesenchymal stem cells from the compact bone of the spiny mouse have been isolated and characterized. Such methodological approaches overcome the lack of species-specific antibodies for the spiny mouse and could be employed for other species where the cost of generating species-specific antibodies is not warranted.

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Acknowledgments

HD and GJ are supported by research funding from the Victorian Government’s Operational Infrastructure Support Program. HD is an Australian Research Council APD Research Fellow.

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Authors and Affiliations

  1. Monash Immunology and Stem Cell Laboratories (MISCL), Melbourne, VIC, Australia

    Hayley Dickinson, Phillipa Milton & Graham Jenkin

  2. The Ritchie Centre, Monash Institute of Medical Research, Clayton, VIC, Australia

    Hayley Dickinson & Graham Jenkin

Authors
  1. Hayley Dickinson
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  2. Phillipa Milton
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  3. Graham Jenkin
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Corresponding author

Correspondence to Hayley Dickinson.

Additional information

Hayley Dickinson and Phillipa Milton contributed equally to this study.

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Dickinson, H., Milton, P. & Jenkin, G. The isolation and characterization of putative mesenchymal stem cells from the spiny mouse. Cytotechnology 64, 591–599 (2012). https://doi.org/10.1007/s10616-012-9443-4

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  • DOI: https://doi.org/10.1007/s10616-012-9443-4

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