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Expansion of human hematopoietic stem cells for transplantation: trends and perspectives

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Abstract

Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated in several ways such as static cultures combining growth factors or mimicking the natural microenvironment using co-culture systems. However, static cultures have a small volume capacity and therefore large-scale expansion has been addressed using bioreactors. These and other biotechnological approaches for the expansion of hematopoietic progenitors and their utility to study several aspects of hematopoietic stem cell biology are discussed here.

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Acknowledgements

This work was partially supported by CONACYT-Fondos Sectoriales Salud Grant 13993 and Grant I0010-2007-01, the sabbatical fellowship 79973 and PhD scholarship 103403.

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  1. División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055 Col. Lomas 4 Sección, CP 78216, San Luis Potosi, S.L.P., Mexico

    Hera Andrade-Zaldívar, Leticia Santos & Antonio De León Rodríguez

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  1. Hera Andrade-Zaldívar
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  2. Leticia Santos
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Correspondence to Antonio De León Rodríguez.

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Andrade-Zaldívar, H., Santos, L. & De León Rodríguez, A. Expansion of human hematopoietic stem cells for transplantation: trends and perspectives. Cytotechnology 56, 151–160 (2008). https://doi.org/10.1007/s10616-008-9144-1

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