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Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

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Abstract

Biosorption of actinides like uranium by fungal cells can play an important role in the mobilization or immobilization of these elements in nature. Sorption experiments of U(VI) with Schizophyllum commune at different initial uranium concentrations and varying metal speciation showed high uranium sorption capacities in the pH range of 4–7. A combination of high angle annular dark-field and scanning transmission electron microscopy analysis (HAADF-STEM) showed that living mycelium cells accumulate uranium at the cell wall and intracellular. For the first time the fluorescence properties of uranium accumulates were investigated by means of time-resolved laser-induced fluorescence spectroscopy (TRLFS) beside the determination of corresponding structural parameters using X-ray absorption fine structure spectroscopy (EXAFS). While the oxidation state of uranium remained unchanged during sorption, uranium speciation changed significantly. Extra and intracellular phosphate groups are mainly responsible for uranium binding. TRLFS spectra clearly show differences between the emission properties of dissolved species in the initial mineral medium and of uranium species on fungi. The latter were proved to be organic and inorganic uranyl phosphates formed depending on the uranyl initial concentration and in some cases on pH.

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Acknowledgments

This work was financially supported by the BMBF under contract No. 0258517 and Grants CGL2009-09760 and CGL2012 (Ministerio de Ciencia e Innovación, Spain). The authors thank for the experimental support during the EXAFS measurements by the ROBL group members A. Scheinost and C. Henning at the ESRF in Grenoble, France. We also thank R. Vochten (University of Antwerp) for providing the m-autunite sample and U. Schaefer, A. Ritter and C. Eckardt for the realization of different chromatographic and ICP-MS measurements. We acknowledge the assistance at the HAADF-STEM measurements of Maria del Mar Abad Ortega, Isabel Guerra Tschuschke, Concepcion Hernandez Castillo (Electron Microscopy Services), University of Granada, Spain.

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Authors and Affiliations

  1. Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, P.O. Box 510119, 01314, Dresden, Germany

    Alix Günther, Johannes Raff, André Roßberg & Gert Bernhard

  2. Departamento de Microbiologia, Universidad de Granada, 18071, Granada, Spain

    Mohamed Larbi Merroun

  3. Institute of Microbiology, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany

    Erika Kothe

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  1. Alix Günther
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Correspondence to Alix Günther.

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Günther, A., Raff, J., Merroun, M.L. et al. Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods. Biometals 27, 775–785 (2014). https://doi.org/10.1007/s10534-014-9772-1

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