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Fontibacillus phaseoli sp. nov. isolated from Phaseolus vulgaris nodules

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Abstract

A bacterial strain, designated BAPVE7BT, was isolated from root nodules of Phaseolus vulgaris in Spain. Phylogenetic analysis based on its 16S rRNA gene sequence placed the isolate into the genus Fontibacillus with Fontibacillus panacisegetis KCTC 13564T its closest relative with 97.1 % identity. The isolate was observed to be a Gram-positive, motile and sporulating rod. The catalase test was negative and oxidase was weak. The strain was found to reduce nitrate to nitrite and to produce β-galactosidase but the production of gelatinase, caseinase, urease, arginine dehydrolase, ornithine or lysine decarboxylase was negative. Acetoin production and aesculin hydrolysis were found to be positive. Growth was observed to be supported by many carbohydrates and organic acids as carbon source. MK-7 was identified as the predominant menaquinone and the major fatty acid (43.7 %) as anteiso-C15:0, as occurs in the other species of the genus Fontibacillus. Strain BAPVE7BT displayed a complex lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, four glycolipids, four phospholipids, two lipids, two aminolipids and an aminophospholipid. Mesodiaminopimelic acid was detected in the peptidoglycan. The G+C content was determined to be 45.6 mol% (Tm). Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain BAPVE7BT should be considered a new species of genus Fontibacillus, for which the name Fontibacillus phaseoli sp. nov. is proposed (type strain, LMG 27589T, CECT 8333T).

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Acknowledgments

This work was supported by MINECO (Spanish Central Government) Grant INNPACTO IPT-2011-1283-060000. JDFF is recipient of a contract supported by this project. MHRB is recipient of a JAE-Doc researcher contract from CSIC cofinanced by ERDF.

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Authors and Affiliations

  1. Departamento de Microbiología y Genética, Lab. 209, Universidad de Salamanca, Edificio Departamental de Biología, Campus Miguel de Unamuno, 37007, Salamanca, Spain

    José David Flores-Félix, Raúl Rivas & Encarna Velázquez

  2. Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, León, Spain

    Rebeca Mulas & María José Cuesta

  3. Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain

    Martha-Helena Ramírez-Bahena, María José Cuesta, Alvaro Peix & Encarna Velázquez

  4. Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain

    Martha-Helena Ramírez-Bahena, Javier Brañas, Daniel Mulas & Alvaro Peix

  5. Fertiberia SA, Madrid, Spain

    Fernando González-Andrés

Authors
  1. José David Flores-Félix
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  2. Rebeca Mulas
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  3. Martha-Helena Ramírez-Bahena
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  4. María José Cuesta
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  5. Raúl Rivas
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  6. Javier Brañas
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  7. Daniel Mulas
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  8. Fernando González-Andrés
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  9. Alvaro Peix
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  10. Encarna Velázquez
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Corresponding author

Correspondence to Encarna Velázquez.

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Flores-Félix, J.D., Mulas, R., Ramírez-Bahena, MH. et al. Fontibacillus phaseoli sp. nov. isolated from Phaseolus vulgaris nodules. Antonie van Leeuwenhoek 105, 23–28 (2014). https://doi.org/10.1007/s10482-013-0049-4

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