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Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation

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Abstract

DNA binding with One Finger (Dof) protein is a plant-specific transcription factor implicated in the regulation of many important plant-specific processes, including photosynthesis and carbohydrate metabolism. This study has identified 31 Dof genes (TaDof) in bread wheat through extensive analysis of current nucleotide databases. Phylogenetic analysis suggests that the TaDof family can be divided into four clades. Expression analysis of the TaDof family across all major organs using quantitative RT-PCR and searches of the wheat genome array database revealed that the majority of TaDof members were predominately expressed in vegetative organs. A large number of TaDof members were down-regulated by drought and/or were responsive to the light and dark cycle. Further expression analysis revealed that light up-regulated TaDof members were highly correlated in expression with a number of genes that are involved in photosynthesis or sucrose transport. These data suggest that the TaDof family may have an important role in light-mediated gene regulation, including involvement in the photosynthetic process.

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Acknowledgements

This work was financially supported by the University of Queensland, the Grains Research and Development Corporation (GRDC) and the Commonwealth Scientific and Industrial Research Organisation (CSIRO). We are also grateful to Dr. Rosanne Casu for her help in phylogenetic analysis.

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Authors and Affiliations

  1. CSIRO Plant Industry, 306 Carmody Rd, St Lucia, QLD, 4067, Australia

    Lindsay M. Shaw, C. Lynne McIntyre & Gang-Ping Xue

  2. ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St Lucia, QLD, 4072, Australia

    Lindsay M. Shaw & Peter M. Gresshoff

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  1. Lindsay M. Shaw
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  2. C. Lynne McIntyre
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Correspondence to Gang-Ping Xue.

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Shaw, L.M., McIntyre, C.L., Gresshoff, P.M. et al. Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation. Funct Integr Genomics 9, 485–498 (2009). https://doi.org/10.1007/s10142-009-0130-2

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