Abstract
Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na2[VO(silibinin)2]·6H2O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments.
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Beecher GR (2003) J Nutr 133:3248S–3254S
Ferrer EG, Williams PAM (2011) Modification of flavonoid structure by oxovanadium(IV) complexation. Biological effects. In: Yamane K, Kato Y (eds) Handbook on flavonoids: dietary sources, properties and health benefits. Nova, Hauppauge, pp 145–190
Kima JD, Liub L, Guob W, Meydani M (2006) J Nutr Biochem 17:165–176
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Int J Biochem Cell Biol 39:44–84
Gazák R, Walterova D, Kren V (2007) Curr Med Chem 14:315–338
Svagera Z, Skottová N, Vána P, Vecera R, Urbánek K, Belejová M, Kosina P, Simánek V (2003) Phytother Res 17:524–530
Varga Z, Czompa A, Kakuk G, Antus A (2001) Phytother Res 15:608–612
Ge Y, Zhang Y, Chen Y, Li Q, Chen J, Dong Y, Shi W (2011) Int J Mol Sci 12:4861–4871
Agarwal C, Wadhwa R, Deep G, Biedermann D, Gažák R, Křen V, Agarwal R (2013) PLoS One 8:e60074. doi:10.1371/journal.pone.0060074
Singh RP, Agarwal R (2004) Curr Cancer Drug Targets 4:1–11
Mokhtari MJ, Motamed N, Shokrgozar MA (2008) Cell Biol Int 32:888–892
Bhatia N, Zhao J, Wolf DM, Agarwala R (1999) Cancer Lett 147:77–84
Hogan FS, Krishnegowda NK, Mikhailova M, Kahlenberg MS (2007) J Surg Res 143:58–65
Kauntz H, Bousserouel S, Gosse F, Marescaux J, Raul F (2012) Int J Oncol 41:849–854
Kauntz H, Bousserouel S, Gosse F, Raul F (2013) Oncol Lett 5:1273–1277
Naso LG, Ferrer EG, Butenko N, Cavaco I, Lezama L, Rojo T, Etcheverry SB, William PAM (2011) J Biol Inorg Chem 16:653–668
Kuntić V, Filipović I, Vujić Z (2011) Molecules 16:1378–1388
Leon IE, Di Virgilio AL, Porro V, Muglia CI, Naso LG, Williams PAM, Bollati-Fogolin M, Etcheverry SB (2013) Dalton Trans 42:11868–11880
Nielsen FH (1995) In: Sigel H, Sigel A (eds) Metal ions in biological systems, vanadium and its role in life. Dekker, New York, pp 543–574
Slebodnicj C, Hamstra BJ, Pecoraro VL (1997) Struct Bonding 89:51–107
Srivastava K, Mehdi MZ (2004) Diabetic Med 22:2–13
Cortizo AM, Etcheverry SB (1995) Mol Cell Biochem 145:97–102
Etcheverry SB, Barrio DA (2007) In: Kustin K, Costa Pesoa J, Crans DC (eds) Vanadium: the versatile metal. ACS symposium series, vol 974. American Chemical Society, Washington, pp 204–216
Djordjevic C, Wampler GL (1985) J Inorg Biochem 25:51–55
Evangelou AM (2002) Crit Rev Oncol Hematol 42:249–265
Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD (2002) Am J Med 113:71–88
Bhuiyan MS, Fukunaga K (2009) J Pharmacol Sci 110:1–13
Shioda N, Han F, Fukunaga K (2009) Int Rev Neurobiol 85:375–387
Etcheverry SB, Williams PAM (2009) New developments in medicinal chemistry. In: Ortega MP, Gil IC (eds) Medicinal chemistry of copper and vanadium bioactive compounds. Nova, Hauppauge, pp 105–129
Barrio DA, Etcheverry SB (2010) Curr Med Chem 17:3632–3642
Djordjevic C (1995) In: Sigel H, Sigel A (eds) Metal ions in biological systems, vanadium and its role in life. Dekker, New York, pp 595–616
Mohseny AB, Pancras CW, Hogendoorn CW, Cleton-Jansen AM (2012) Sarcoma. doi:10.1155/2012/417271
Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C (1991) J Immunol Methods 139:271–280
Okajima T, Nakamura H, Zhang Y, Ling N, Tanabe T, Yasuda T, Rosenfeld RG (1992) Endocrinology 130:2201–2212
Borenfreund E, Puerner JA (1984) J Tissue Cult Methods 9:7–9
Mosmann T T (1983) J Immunol Methods 65:55–63
Fenech M (2000) Mutat Res 455:81–95
Fenech M (1993) Mutat Res 1:35–44
Singh NP, McCoy MT, Tice RR, Schneider EL (1988) Exp Cell Res 1988(175):184–191
Cortizo AM, Bruzzone L, Molinuevo MS, Etcheverry SB (2000) Toxicology 147:89–99
Hissin PJ, Hilf R (1976) Anal Biochem 74:214–226
Bradford M (1976) Anal Biochem 72:248–254
Hurley AA (2001) Curr Protoc Cytom 7.2.1–7.2.5
Pozarowski P, Grabarek J, Darzynkiewicz Z (2003) Curr Protoc Cytom 25:7.19.1–7.19.33
Stopper H, Muller SO (1997) Toxicol In Vitro 11:661–667
Collins AR, Dobson VL, Dusinka M, Kennedy G, Stetina R (1997) Mut Res 375:183–193
Liao W, Nutt MA, Zhu MG (2009) Methods 48:46–53
Leon IE, Di Virgilio AL, Barrio DA, Arrambide G, Gambino D, Etcheverry SB (2012) Metallomics 4:1287–1296
Rivadeneira J, Di Virgilio AL, Barrio DA, Muglia CI, Bruzzone L, Etcheverry SB (2010) Med Chem 6:9–23
Ye J, Ding M, Leonard SS, Robinson VA, Millecchia L, Zhang X, Castranova V, Vallyathan V, Shi X (1999) Mol Cell Biochem 202:9–17
Zhang Z, Huang C, Li J, Leonard SS, Lanciotti R, Butterworth L, Shi X (2001) Arch Biochem Biophys 392:311–332
Capella MAM, Capella LS, Valente RC, Gefe′ M, Lopes AG (2007) Cell Biol Toxicol 23:413–420
Jones DP, Carlson JL, Mody VC, Cai JY, Lynn MJ, Sternberg P (2000) Free Radic Biol Med 28:625–635
Hwang C, Sinskey AJ, Lodish HF (1992) Science 57:1496–1502
Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M, Gareau Y, Griffin PR, Labelle M, Lazebnik YA (1995) Nature 376:37–43
Sakahira H, Enari M, Nagata S (1998) Nature 391:96–99
Kamada S, Kusano H, Fujita H, Ohtsu M, Koya RC, Kuzumaki N, Tsujimoto Y (1998) Proc Natl Acad Sci USA 95:8532–8537
Porter AG, Janicke RU (1999) Cell Death Differ 6:99–104
Sherr CJ (2000) Cancer Res 60:3695–3698
Kerr JFR, Wyllie AH, Curie AR (1972) Br J Cancer 26:239–257
Arends MJ, Morris RG, Wyllie AH (1990) Am J Pathol 136:593–608
Nagata S (2000) Exp Cell Res 256:12–18
Umansky SR, Korol BA, Nelipovich PA (1981) Biochim Biophys Acta 655:9–17
Ottaviani G, Jaffe N (2009) The epidemiology of osteosarcoma. In: Jaffe N et al (eds) Pediatric and adolescent osteosarcoma. Springer, New York
Strianese M, Basile A, Mazzone A, Morello S, Turco MC, Pellecchia C (2013) J Cell Physiol 228:2202–2209
Rodriguez-Mercado JJ, Mateos-Nava RA, Altamirano-Lozano MA (2011) Toxicol In Vitro 25:1996–2002
Di Virgilio AL, Rivadeneira J, Muglia CI, Reigosa MA, Butenko N, Cavaco I, Etcheverry SB (2011) Biometals 24:1153–1168
Villani P, Cordelli PE, Leopardo P, Siniscalchi E, Veschetti E, Fresegna AM, Crebelli R (2007) Toxicol Lett 170:11–18
Wang CC, Chiang YM, Sung SC, Hsu YL, Chang JK, Kuo PL (2008) Cancer Lett 259:82–98
Baumgartner HK, Gerasimenko JV, Thorne C, Ashurst LH, Barrow SL, Chvanov MA, Gillies S, Criddle DN, Tepikin AV, Petersen OH, Sutton R, Watson AJM, Gerasimenko OV (2007) Am J Physiol Gastrointest Liver Physiol 293:296–307
Valko M, Morris H, Cronin MT (2005) Curr Med Chem 12:1161–1208
Saxena AK, Srivastava P, Kale RK, Baquer NZ (1993) Biochem Pharmacol 45:539–542
Sabbioni E, Pozzi G, Devos S, Pintar A, Casella L, Fischbach M (1993) Carcinogenesis 14:2565–2568
Gonçalves AP, Videira A, Soares P, Maximo V (2011) Life Sci 12:11–12
Montiel-Davalos A, Gonzalez-Villava A, Rodriguez-Lara V, Montano LF, Fortoul TI, Lopez-Marure R (2012) J Appl Toxicol 32:26–33
Zhao Y, Ye L, Liu H, Xia Q, Zhang Y, Yang X, Wang K (2010) J Inorg Biochem 104:371–378
Rivadeneira J, Barrio DA, Arrambide G, Gambino D, Bruzzone L, Etcheverry SB (2009) J Inorg Biochem 103:633–642
Hosseini MJ, Seyedrazi N, Shahraki J, Pourahmad J (2012) Adv Biosci Biotechnol 3:1096–1103
Afeseh Ngwa H, Kanthasamy A, Anantharam V, Song C, Witte T, Houk R, Kanthasamy AG (2009) Toxicol Appl Pharmacol 240:273–285
Ray RS, Rana B, Swami B, Venu V, Chatterjee M (2006) Chem Biol Interact 163:239–247
Fu Y, Wang Q, Yang XG, Yang XD, Wang K (2008) J Biol Inorg Chem 13:1001–1009
Acknowledgments
This work was partly supported by UNLP (11X/554), CONICET (PIP 1125), and ANPCyT (PICT 2008-2218 and PICT-2010-0981) from Argentina. A.L.D.V.and S.B.E. are members of the Carrera del Investigador, CONICET, Argentina. P.A.M.W. is member of the Carrera del Investigador, CIC, and PBA. I.E.L. has a fellowship from ANPCyT, Argentina, and a fellowship from AMSUD- PASTEUR, Institut Pasteur, Uruguay. L.G.N. has a postdoctoral fellowship from CONICET, Argentina. V.P. and M.B.F. are members of the Sistema Nacional de Investigadores of the Agencia Nacional de Investigación e Innovación in Uruguay. The authors would like to thank Inés Tiscornia for the management work with the cells and Miguel Reigosa for help with the PMBC assay. Moreover, the authors would like to thank M.C. Bernal for her careful revision of the manuscript.
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Leon, I.E., Porro, V., Di Virgilio, A.L. et al. Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells. J Biol Inorg Chem 19, 59–74 (2014). https://doi.org/10.1007/s00775-013-1061-x
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DOI: https://doi.org/10.1007/s00775-013-1061-x