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Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)

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Abstract

A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.

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Acknowledgments

We thank to Dr. E. Johansen for providing plasmid pAGUS1 and her helpful advice, Dr. C. Nome for her help with the electron microscopy techniques, and Dr. E. Taleisnik for editing the manuscript.

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Authors and Affiliations

  1. Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino 60 cuadras Km 5,5, X5020ICA, Córdoba, Argentina

    N. Bejerman, F. Giolitti, S. de Breuil & S. Lenardon

  2. Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Córdoba, Argentina

    S. Lenardon

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  1. N. Bejerman
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  2. F. Giolitti
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  3. S. de Breuil
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  4. S. Lenardon
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Corresponding author

Correspondence to N. Bejerman.

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Supplementary material 1 (DOC 30 kb)

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705_2012_1497_MOESM3_ESM.tif

Supplementary Figure 1: Nucleotide sequence alignment of p35SucMoV and SuCMoV-C. Silent mutations are highlighted in green, whereas differences that resulted in aa substitutions are highlighted in red. One of the 15 differences (located at nt position 5098) is not shown in the figure because the last 8 nucleotides of each row are not shown. Therefore, a .txt file with the alignment has been uploaded as an annex file of this figure.(TIFF 2069 kb)

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Bejerman, N., Giolitti, F., de Breuil, S. et al. Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV). Arch Virol 158, 485–490 (2013). https://doi.org/10.1007/s00705-012-1497-2

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  • DOI: https://doi.org/10.1007/s00705-012-1497-2

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