Abstract
The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant.
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Acknowledgments
SW was supported by an Australian Research Council Linkage Grant (LP0882671), funded partially by industry partners Alcoa Australia Pty Ltd, BHP Billiton Worsley Alumina Pty Ltd, Kings Park and Botanic Garden, Department of Agriculture and Food Western Australia, and the Murdoch University Institutes for Crop and Plant Science Research and Sustainable Ecosystems Research.
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Wylie, S.J., Jones, M.G.K. The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses. Arch Virol 156, 479–482 (2011). https://doi.org/10.1007/s00705-010-0845-3
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DOI: https://doi.org/10.1007/s00705-010-0845-3