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Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization

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Abstract

 Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.

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Authors and Affiliations

  1. Laboratoire de Phytoparasitologie INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement, INRA, BV 1540, F-21034 Dijon Cedex, France e-mail : gianina@epoisses.inra.fr, Fax: +333-80-63-32-63, , , , , , FR

    Sophie Trouvelot, Diederik van Tuinen, Mohamed Hijri & V. Gianinazzi-Pearson

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  1. Sophie Trouvelot
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  2. Diederik van Tuinen
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  3. Mohamed Hijri
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  4. V. Gianinazzi-Pearson
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Accepted: 16 September 1998

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Trouvelot, S., van Tuinen, D., Hijri, M. et al. Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization. Mycorrhiza 8, 203–206 (1999). https://doi.org/10.1007/s005720050235

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  • DOI: https://doi.org/10.1007/s005720050235

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