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Detection and identification of Borrelia burgdorferi sensu lato genospecies in ticks from three different regions in Slovakia

Nachweis und Identifizierung von Borrelia burgdorferi sensu lato-Genospezies in Zecken aus drei verschiedenen Regionen der Slowakei

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Zusammenfassung

Lyme-Borreliose ist eine der häufigsten von Zecken vermittelten Erkrankungen, die in der Slowakei auftreten. In dieser Studie wurde Borrelia burgdorferi sensu lato in Wirt-suchenden Zecken, die aus drei Regionen der Slowakei gesammelt worden sind, nachgewiesen und kultiviert. Zur Identifizierung der Genospezies wurden zwei Methoden eingesetzt, die RFLP (restriction fragment length polymorphism) und der "reverse line blot". Ebenso wurde die Prävalenz von B. burgdorferi s.l. in den Zecken ermittelt, welche rund 32% betrug. Von den vier identifizierten Genospezies, B. afzelii, B. burgdorferi sensu stricto, B. garinii und B. valaisiana, wurde B. garinii am häufigsten nachgewiesen.

Summary

Lyme borreliosis is one of the most common tick-borne diseases that occur in Slovakia. In this study, Borrelia burgdorferi sensu lato was detected and cultivated from questing ticks collected in three areas of Slovakia. Two methods, restriction fragment length polymorphism and reverse line blot, were used for identification of isolates and determination of the prevalence of B. burgdorferi s.l. in the ticks. The prevalence of B. burgdorferi s.l. in I. ricinus detected by reverse line blot was 31.9%. Four genospecies, namely B. garinii, B. valaisiana, B. afzelii and B. burgdorferi sensu stricto were found. B. garinii was the most prevalent genospecies.

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Authors and Affiliations

  1. Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland

    Caroline Burri, David Pérez & Lise Gern

  2. Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia

    Katarína Smetanová & Elena Kocianová

Authors
  1. Katarína Smetanová
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  2. Caroline Burri
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  3. David Pérez
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  4. Lise Gern
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  5. Elena Kocianová
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Correspondence to Elena Kocianová.

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Smetanová, K., Burri, C., Pérez, D. et al. Detection and identification of Borrelia burgdorferi sensu lato genospecies in ticks from three different regions in Slovakia. Wien Klin Wochenschr 119, 534–537 (2007). https://doi.org/10.1007/s00508-007-0851-y

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