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Optimization of lipase-catalyzed glucose ester synthesis in ionic liquids

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Abstract

Lipase-catalyzed esterification of glucose with fatty acids in ionic liquids (ILs) mixture was investigated by using supersaturated glucose solution. The effect of ILs mixture ratio, substrate ratio, lipase content, and temperature on the activity and stability of lipase was also studied. The highest yield of sugar ester was obtained in a mixture of 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TfO]) and 1-methyl-3-octylimidazolium bis[(trifluoromethyl)-sulfonyl]amide ([Omim][Tf2N]) with a volume ratio of 9:1, while Novozym 435 (Candida antarctica type B lipase immobilized on acrylic resin) showed the optimal stability and activity in a mixture of [Bmim][TfO] and [Omim][Tf2N] with a 1:1 volume ratio. Reuse of lipase and ILs was successfully carried out at the optimized reaction conditions. After 5 times reuse of Novozym 435 and ILs, 78% of initial activity was remained.

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Abbreviations

[Bmim][TfO]:

1-Butyl-3-methylimidazolium trifluoromethanesulfonate

[Bmim][Tf2N]:

1-Butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide

[Omim][Tf2N]:

1-Methyl-3-octylimidazolium bis[(trifluoromethyl)sulfonyl]amide

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Acknowledgments

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-313-D00170). This work was also supported in part by the Engineering Research Center for Advanced Bioseparation Technology, Inha University. The authors also would like to acknowledge to Minjeong Koo for her help.

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Authors and Affiliations

  1. ERC for Advanced Bioseparation Technology, Inha University, Incheon, 402-751, Korea

    Sung Ho Ha & Yoon-Mo Koo

  2. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Nguyen Minh Hiep & Yoon-Mo Koo

  3. Department of Microbial Engineering, Konkuk University, Seoul, 143-701, Korea

    Sang Hyun Lee

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  1. Sung Ho Ha
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  2. Nguyen Minh Hiep
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  3. Sang Hyun Lee
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  4. Yoon-Mo Koo
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Correspondence to Sung Ho Ha or Yoon-Mo Koo.

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Ha, S.H., Hiep, N.M., Lee, S.H. et al. Optimization of lipase-catalyzed glucose ester synthesis in ionic liquids. Bioprocess Biosyst Eng 33, 63–70 (2010). https://doi.org/10.1007/s00449-009-0363-4

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  • DOI: https://doi.org/10.1007/s00449-009-0363-4

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