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Allele frequency distribution of 10 MiniSTRs in the Pashtun population of Pakistan

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Abstract

Two hundred individual samples of Pashtun population from Khyber Pakhtunkhwa province of Pakistan were randomly evaluated through 10 MiniSTR loci (CSF1PO, D7S820, TPOX, D18S51, D2S1338, D13S317, FGA, D5S818, D21S11, and D16S539). The PCR product size was reduced in the range of 65 to 280 bp. A total of 112 alleles were observed containing allelic frequency ranging from 0.0025 to 0.4325. Statistical values for forensic and parentage analysis were calculated including combined power of discrimination (PD), combined power of exclusion (PE), and cumulative probability of matching (PM) and equaled to 0.99999999999768, 0.99984944, and 2.33 × 10−12, respectively. These MiniSTRs show a high degree of polymorphism information content and discriminatory power which would be helpful to resolve forensic cases and establish DNA database for major population groups of Pakistan. In contrast to different populations, significant differences were also observed on these loci.

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Authors and Affiliations

  1. Forensic DNA Typing Laboratory, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan

    Muhammad Shafique, Uzma Perveen, Ziaur Rehman, Ahmad Ali Shahid & Tayyab Husnain

  2. Forensic Science Section, Centre for Research in Molecular Medicine, The University of Lahore, Lahore, Pakistan

    Muhammad Saqib Shahzad & Azam Ali

  3. Forensic Services Laboratory, Centre for Applied Molecular Biology, Ministry of Science and Technology, Lahore, 53700, Pakistan

    Rukhsana Parveen & Manzoor Hussain

Authors
  1. Muhammad Shafique
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  2. Muhammad Saqib Shahzad
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  3. Uzma Perveen
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  4. Rukhsana Parveen
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  5. Azam Ali
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  6. Manzoor Hussain
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  7. Ziaur Rehman
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  8. Ahmad Ali Shahid
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  9. Tayyab Husnain
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Corresponding author

Correspondence to Muhammad Saqib Shahzad.

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Shafique, M., Shahzad, M.S., Perveen, U. et al. Allele frequency distribution of 10 MiniSTRs in the Pashtun population of Pakistan. Int J Legal Med 129, 467–468 (2015). https://doi.org/10.1007/s00414-015-1179-1

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  • DOI: https://doi.org/10.1007/s00414-015-1179-1

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