Abstract
Candida glycerinogenes, a glycerol production industrial strain with hyperosmo-adaptation can grow well in 15 % (w/v) NaCl or 55 % (w/v) glucose. To understand the osmo-adaptation mechanism in C. glycerinogenes, the mitogen-activated protein kinase HOG1 gene (CgHOG1), which plays an essential role in the yeast hyperosmotic response, was isolated by degenerate PCR and SEFA-Formed Adaptor PCR. The CgHOG1 gene was then transformed in Saccharomyces cerevisiae hog1Δ null mutant, which restored the recombination S. cerevisiae to the wild-type phenotype with osmo-adaptation. To further clarify the function of CgHOG1, the phosphorylation of CgHOG1 and transcription of the glycerol-3-phosphate dehydrogenase gene (GPD1) of the CgHOG1-harbouring S. cerevisiae mutant was detected, and found to be similar to that of wild-type S. cerevisiae. In addition, the recombination S. cerevisiae with CgHOG1 gene significantly accumulated intracellular glycerol when stressed with NaCl.
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This work was supported by the National High Technology Research and Development Program of China (863 Program, NO.2011AA02A207, NO.2012AA021201), Natural Science Foundation of China (NO.31270080), Natural Science Foundation of Jiangsu Province (No. BK20140138), Jiangnan University Independent Scientific Research Program (No. JUSRP1008, No. JUSRP11431) and the 111 Project (No. 111-2-06).
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Hao Ji and Xinyao Lu contributed equally to this study and share first authorship.
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Ji, H., Lu, X., Wang, C. et al. Identification of a Novel HOG1 Homologue from an Industrial Glycerol Producer Candida glycerinogenes . Curr Microbiol 69, 909–914 (2014). https://doi.org/10.1007/s00284-014-0670-0
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DOI: https://doi.org/10.1007/s00284-014-0670-0