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Impact of increasing NaCl concentrations on the performance and community composition of two anaerobic reactors

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Abstract

The anaerobic treatment of saline effluents using halophilic and halotolerant microbial consortia is of major interest. Inhibition of anaerobic digestion is known to occur at high salt content. However, it seems that the suitable adaptation of an anaerobic sludge makes possible the treatment of saline wastewater. In this study, a non-saline anaerobic sludge was inoculated in two anaerobic batch reactors operating with a different substrate (distillery vinasse and ethanol) and subjected to increasing NaCl concentrations. The performance of the digesters appeared to be highly dependent on the nature of the substrate, and a similar level of inhibition (i.e. around 90% of the specific loading rate and specific methanogenic activity) was stated at 10 g l−1 of NaCl with distillery vinasse and 60 g l−1 of NaCl with ethanol. The characterization of the microflora and its adaptation to increasing NaCl conditions were also investigated using molecular tools based on the analysis of genomic 16S rDNA. The microbial communities revealed a high diversity that could be maintained in both reactors despite the increase in NaCl concentrations.

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References

  • AFNOR (1997) Qualité de l’eau, recueil des normes françaises. AFNOR, Paris

    Google Scholar 

  • Aspé E, Martí MC, Jara A, Roeckel M (2001) Ammonia inhibition in the anaerobic treatment of fishery effluents. Water Environ Res 73:154–164

    Article  Google Scholar 

  • Boardman GD, Tisinger JL, Gallagher DL (1995) Treatment of clam processing wastewaters by means of upflow anaerobic sludge blanket technology. Water Res 29:1483–1490

    Article  CAS  Google Scholar 

  • Feijoo G, Soto M, Mendez R, Lema JM (1995) Sodium inhibition in the anaerobic digestion process: antagonism and adaptation phenomena. Enzyme Microb Technol 17:180–188

    Article  CAS  Google Scholar 

  • Gangagni Rao A, Venkata Naidu G, Krishna Prasad K, Chandrasekhar Rao N, Venkata Mohan S, Jetty A, Sarma PN (2005) Anaerobic treatment of wastewater with high suspended solids from a bulk drug industry using fixed film reactor (AFFR). Bioresour Technol 96:87–93

    Article  CAS  Google Scholar 

  • Gebauer R (2004) Mesophilic anaerobic treatment of sludge from saline fish farm effluents with biogas production. Bioresour Technol 93:155–167

    Article  CAS  Google Scholar 

  • Godon JJ, Zumstein E, Dabert P, Habouzit F, Moletta R (1997a) Microbial 16S rDNA diversity in an anaerobic digester. Water Sci Technol 36:49–55

    Article  CAS  Google Scholar 

  • Godon JJ, Zumstein E, Dabert P, Habouzit F, Moletta R (1997b) Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Appl Environ Microbiol 63:2802–2813

    Article  CAS  Google Scholar 

  • Gourdon R, Comel C, Vermande P, Veron J (1989) Kinetics of acetate, propionate and butyrate removal in the treatment of a semi-synthetic landfill leachate on anaerobic filter. Biotechnol Bioeng 33:1167–1181

    Article  CAS  Google Scholar 

  • Guerrero L, Omil F, Méndez R, Lema JM (1997) Treatment of saline wastewaters from fish meal factories in an anaerobic filter under extreme ammonia concentrations. Bioresour Technol 61:69–78

    Article  CAS  Google Scholar 

  • Habets LHA, Engelaar AJHH, Groeneveld N (1997) Anaerobic treatment of inuline effluent in an internal circulation reactor. Water Sci Technol 35:189–197

    Article  CAS  Google Scholar 

  • Henze M, Harremoës P (1983) Anaerobic treatment of wastewaters in fixed film reactors—A literature review. Water Sci Technol 15:1–101

    Article  CAS  Google Scholar 

  • Kargi F, Dincer AR (1996) Effect of salt concentration on biological treatment of saline wastewater by fed-batch operation. Enzyme Microb Technol 19:529–537

    Article  CAS  Google Scholar 

  • Kugelman IJ, McCarty PL (1965) Cation toxicity and stimulation in anaerobic waste treatment. I. Slug feed studies. J Water Pollut Control Fed 37:97–116

    CAS  Google Scholar 

  • Lefebvre O, Habouzit F, Bru V, Delgenes JP, Godon JJ, Moletta R (2004) Treatment of hypersaline industrial wastewater by a microbial consortium in a sequencing batch reactor. Environ Technol 25:543–553

    Article  CAS  Google Scholar 

  • Lefebvre O, Vasudevan N, Torrijos M, Thanasekaran K, Moletta R (2005) Halophilic biological treatment of tannery soak liquor in a sequencing batch reactor. Water Res 39:1471–1480

    Article  CAS  Google Scholar 

  • Lefebvre O, Vasudevan N, Torrijos M, Thanasekaran K, Moletta R (2006a) Anaerobic digestion of tannery soak liquor with an aerobic post-treatment. Water Res 40:1492–1500

    Article  CAS  Google Scholar 

  • Lefebvre O, Vasudevan N, Thanasekaran K, Moletta R, Godon JJ (2006b) Microbial diversity in hypersaline wastewater: the example of tanneries. Extremophiles 10:505–513

    Article  CAS  Google Scholar 

  • Moon BH, Seo GT, Lee TS, Kim SS, Yoon CH (2003) Effects of salt concentration on floc characteristics and pollutants removal efficiencies in treatment of seafood wastewater by SBR. Water Sci Technol 47:65–70

    Article  CAS  Google Scholar 

  • Mosquera-Corral A, Sanchez M, Campos JL, Mendez R, Lema JM (2001) Simultaneous methanogenesis and denitrification of pretreated effluents from a fish canning industry. Water Res 35:411–418

    Article  CAS  Google Scholar 

  • Ng HY, Ong S, Ng WJ (2005) Effects of sodium chloride on the performance of a sequencing batch reactor. J Environ Eng ASCE 131:1557–1564

    Article  CAS  Google Scholar 

  • Omil F, Mendez R, Lema JM (1995) Anaerobic treatment of saline wastewaters under high sulphide and ammonia content. Bioresour Technol 54:269–278

    Article  CAS  Google Scholar 

  • Rinzema A, Lier Vv, Lettinga G (1988) Sodium inhibition of acetoclastic methanogens in granular sludge from a UASB reactor. Enzyme Microb Technol 10:24–32

    Article  CAS  Google Scholar 

  • Rovirosa N, Sanchez E, Cruz M, Veiga MC, Borja R (2004) Coliform concentration reduction and related performance evaluation of a down-flow anaerobic fixed bed reactor treating low-strength saline wastewater. Bioresour Technol 94:119–127

    Article  CAS  Google Scholar 

  • Ruíz C, Torrijos M, Sousbie P, Lebrato Martínez J, Moletta R, Delgenès JP (2002) Treatment of winery wastewater by an anaerobic sequencing batch reactor. Water Sci Technol 45:219–224

    Article  Google Scholar 

  • Sawyer CN, McCarty PL (1967) Chemistry for sanitary engineers. McGraw-Hill, New York

    Google Scholar 

  • Uygur A, Kargi F (2004) Salt inhibition on biological nutrient removal from saline wastewater in a sequencing batch reactor. Enzyme Microb Technol 34:313–318

    Article  CAS  Google Scholar 

  • Vidal G, Aspé E, Martí MC, Roeckel M, Vidal G (1997) Treatment of recycled wastewaters from fishmeal factory by an anaerobic filter. Biotechnol Lett 19:117–121

    Article  CAS  Google Scholar 

  • Vlyssides AG, Barampouti EM, Mai S (2005) Wastewater characteristics from Greek wineries and distilleries. Water Sci Technol 51:53–60

    Article  CAS  Google Scholar 

  • Woolard CR, Irvine RL (1995) Treatment of hypersaline wastewater in the sequencing batch reactor. Water Res 29:1159–1168

    Article  CAS  Google Scholar 

  • Zumstein E, Moletta R, Godon JJ (2000) Examination of two years of community dynamics in an anaerobic bioreactor using fluorescence polymerase chain reaction (PCR) single-strand conformation polymorphism analysis. Environ Microbiol 2:69–78

    Article  CAS  Google Scholar 

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Lefebvre, O., Quentin, S., Torrijos, M. et al. Impact of increasing NaCl concentrations on the performance and community composition of two anaerobic reactors. Appl Microbiol Biotechnol 75, 61–69 (2007). https://doi.org/10.1007/s00253-006-0799-2

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  • DOI: https://doi.org/10.1007/s00253-006-0799-2

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