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Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix

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Abstract

A composite biosorbent retaining non-covalently bound chitin in between layers of a silicon oxide matrix was assessed for lysozyme purification from undiluted egg white. The matrix can be shaped with big size and high density, thus allowing its efficient separation from the egg white after the adsorption step. The lysozyme-depleted egg white can follow its usual commercialisation route. A surface area of 142 m2/g and a total pore volume of 0.295 cm3/g were calculated from the nitrogen sorption isotherms. Its water content was 78.3%. The matrix structure is the result of the polysaccharide addition to the polymerisation mixture, which is known to influence the condensation process, leading to a material with characteristic properties. A maximum capacity of 117.1 ± 9 mg lysozyme/g and a dissociation constant of 0.73 ± 0.15 mg/mL were calculated from the Langmuir isotherm. A lysozyme purification batch process from undiluted egg white was developed, where 87% of the lysozyme was removed from the egg white and the matrix was easily recovered by a simple filtration through a strainer. The overall yield of the process was 64% with a purification factor of 20.

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Acknowledgments

This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica de la República Argentina, the Universidad de Buenos Aires and the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). FJW, OC, GJC, MVM and LED are researchers of the CONICET.

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Authors and Affiliations

  1. Facultad de Farmacia y Bioquímica, University of Buenos Aires, Junín 956 (1113), Buenos Aires, Argentina

    Federico J. Wolman, Guillermo J. Copello, Andrea M. Mebert, Alexandra M. Targovnik, María V. Miranda, Agustín A. Navarro del Cañizo, Luis E. Díaz & Osvaldo Cascone

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  1. Federico J. Wolman
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  2. Guillermo J. Copello
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  3. Andrea M. Mebert
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  4. Alexandra M. Targovnik
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  5. María V. Miranda
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  6. Agustín A. Navarro del Cañizo
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  7. Luis E. Díaz
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  8. Osvaldo Cascone
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Correspondence to Osvaldo Cascone.

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Wolman, F.J., Copello, G.J., Mebert, A.M. et al. Egg white lysozyme purification with a chitin–silica-based affinity chromatographic matrix. Eur Food Res Technol 231, 181–188 (2010). https://doi.org/10.1007/s00217-010-1263-1

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  • DOI: https://doi.org/10.1007/s00217-010-1263-1

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