Abstract
The U.S. National Institute of Standards and Technology (NIST) has developed and certified a standard reference material, SRM 2382, for use in testing for bias in the determination of morphine present as a glucuronide in human urine. This SRM consists of three levels of morphine-3-β-D-glucuronide (M-3-G) in lyophilized urine. Two independent hydrolysis procedures, enzymatic and acidic, for quantitative release of morphine were investigated. The results of hydrolysis efficiency from these two procedures agreed well. These two hydrolysis procedures along with two analytical methods developed previously for measurement of free morphine [1] were used to certify M-3-G in SRM 2382. Enzymatic hydrolysis was used with the GC/MS method and acidic hydrolysis was used with LC/MS. The results from these two pairs of methods were in good agreement, and were statistically combined to yield certified values of 209 ± 20, 437 ± 21, and 853 ± 39 ng/mL for morphine, as the free base. Round-robin studies on this material among ten military laboratories demonstrated the suitability of the SRM for its intended purpose.
Similar content being viewed by others
References
Tai S, Christensen R, Paule R, Sander L, Welch M (1994) J Anal Toxicol 18: 7–12
Craft N, Byrd G, Hilpert L (1989) Anal Chem 61: 540–544
Ellerbe P, Tai S, Christensen R, Espinosa-Leniz R, Paule R, Sander L, Sniegoski L, Welch M, White E (1992) J Anal Toxicol 16: 158–162
Ellerbe P, Long T, Welch M (1993) J Anal Toxicol 17: 165–170
Tai S, Christensen R, Coakley K, Ellerbe P, Long T, Welch M (1996) J Anal Toxicol 20: 43–49
Federal Register 53(69) 11970-11989 (April 11, 1988)
Welch M, Ellerbe P, Tai S, Christensen R, Sniegoski L, Sander L, Phinney C (1994) Fresenius J Anal Chem 352: 61–65
Boerner U, Abbott S, Roe R (1975) Drug Metab Rev 4: 39–73
Fish F, Hayes T (1974) J Forensic Sci 19: 676–683
Combie J, Blake J, Nugent T, Tobin T (1982) Clin Chem 28: 83–86
Paul B, Mell L, Mitchell J, Irving J, Novak A (1985) J Anal Toxicol 9: 222–226
Jennison T, Wozniak E, Nelson G, Urry F (1993) J Anal Toxicol 17: 208–210
Lin Z, Lafolie P, Beck O (1994) J Anal Toxicol 18: 129–133
Zezulak M, Snyder J, Needleman S (1993) J Forensic Sci 38: 1275–1285
Romberg R, Lee L (1995) J Anal Toxicol 19: 157–162
Schiller S, Eberhardt K (1991) Spectrochimica Acta 46B: 1607–1613
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tai, S.S.C., Christensen, R.G., Sander, L.C. et al. Certification of morphine-3-β-D-glucuronide in a human urine standard reference material. Fresenius J Anal Chem 357, 373–378 (1997). https://doi.org/10.1007/s002160050172
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s002160050172