Abstract
To develop an efficient mustard (Brassica juncea) breeding programme, a better knowledge of the genetic control and relationships of the main selected characters is needed. Thus, doubled haploid (DH) lines were evaluated over 2 years in the field. Days to flowering, plant height, thousand-seed weight, fatty acid composition, seed oil content, sinigrin, gluconapin and total glucosinolate contents were determined in the DH population. The influence of seed coat colour was estimated. Results showed significant differences between yellow and brown seeds only for oil and fatty acid contents. Molecular analysis revealed that seed coat colour is associated with two Mendelian trait loci: Bjc1 [on linkage group (LG) 3] and Bjc2 (on LG6). The quantitative trait loci associated with characters—detected by composite interval mapping—were not co-localised and revealed a genetic independence. The results obtained in this study show that the most important agronomic and quality traits of brown mustard could be bred independently. Correlation between the studied traits is also discussed.
Similar content being viewed by others
References
Abraham V, Bhatia CR (1986) Development of strains with yellow seed coats in Indian mustard (Brassica juncea L. Czern. and Coss.). Plant Breed 97:86–88
Axelsson T, Shavorskaya O, Lagercrantz U (2001) Multiple flowering time QTLs within several Brassica species could be the result of duplicated copies of one ancestral gene. Genome 44:856–864
Basten CJ (1999) QTL Cartographer manual. Available via http://statgen.ncsu.edu/qtlcart/cartographer.html
Bezant J, Laurie D, Pratchett N, Chojecki J, Kearsey M (1996) Marker regression mapping of QTL controlling flowering time and plant height in a spring barley (Hordeum vulgare L.) cross. Heredity 77:64–73
Cheung WY, Landry BS, Raney P, Rakow GFW (1998) Molecular mapping of seed quality traits in Brassica juncea L. Czern. and Coss. In: Gregoire T (ed) Proceedings of international symposium on Brassicas. Acta Hortic 459:139–147
Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Dirlewanger E, Isaac PG, Ranade S, Belajouza M, Cousin R, DeVienne D (1994) Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theor Appl Genet 88:17–27
Foisset N (1995) Elaboration d’une carte génétique du colza (Brassica napus L.)—Marquage de gènes d’intérêt agronomique. Thèse de l’université de Rennes I, p 141
George L, Rao PS (1983) Yellow-seeded variants in in vitro regenerants of mustard (Brassica juncea Coss. var. rai-5). Plant Sci Lett 30:327–330
Gland A, Röbbelen G, Thies W (1981) Variation of alkenyl glucosinolates in seeds of Brassica species. Z Pflanzenzuecht 87:96–110
Halkier BA, Du L (1997) The biosynthesis of glucosinolates. Trends Plant Sci 2:425–431
Josefsson E (1972) Variation of pattern and content of glucosinolates in seed of some cultivated cruciferae. Z Pflanzenzuecht 68:113–123
Kirk JTO, Hurlstone CJ (1983) Variation and inheritance of erucic acid content in Brassica juncea. Z Pflanzenzuecht 90:331–338
Lionneton E, Beuret W, Delaitre C, Ochatt S, Rancillac M (2001) Efficient microspore culture and double haploid plant regeneration for the brown condiment mustard (Brassica juncea). Plant Cell Rep 20:126–130
Lionneton E, Ravera S, Sanchez L, Aubert G, Delourme R, Ochatt S (2002) Development of an AFLP-based linkage map and localisation of QTLs for seed fatty acid content in condiment mustard (Brassica juncea). Genome 45:1203–1215
Love HK, Rakow G, Raney JP, Downey RK (1990) Genetic control of 2-propenyl and 3-butenyl glucosinolate synthesis in mustard. Can J Plant Sci 70:425–429
Magrath R, Bano F, Morgner M, Parkin I, Sharpe A, Lister C, Dean C, Turner J, Lydiate D, Mithen R (1994) Genetics of aliphatic glucosinolates. I. Side chain elongation in Brassica napus and Arabidopsis thaliana. Heredity 72:290–299
Mahmood T, Ekuere U, Yeh F, Good AG, Stringam GR (2003a) RFLP linkage analysis and mapping genes controlling the fatty acid profile of Brassica juncea using reciprocal DH populations. Theor Appl Genet 107:283–290
Mahmood T, Ekuere U, Yeh F, Good AG, Stringam GR (2003b) Molecular mapping of seed aliphatic glucosinolates in Brassica juncea. Genome 46:753–760
Morgan CL, Arthur AE, Rawsthorne S (1998) Influence of testa colour and seed size on storage product composition in Brassica juncea. Plant Varieties Seeds 11:73–81
Mukherjee AK, Mohapatra H, Varshney A, Sharma R, Sharma RP (2001) Molecular mapping of a locus controlling resistance to Albugo candida in Indian mustard. Plant Breed 120:483–487
Negi MS, Devic M, Delseny M, Lakshimikumaran M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101:146–152
Singh JN, Yadav MC, Sheikh IA (1996) Genetical studies for yield and oil content in Brassica juncea (L.) Czern. and Coss. Indian J Genet 56:299–304
Teulat B, Merah O, Souyris I, This D (2001) QTLs for agronomic traits from a Mediterranean barley progeny grown in several environments. Theor Appl Genet 103:774–787
Thiagarajah MR, Stringam GR (1993) A comparison of genetic segregation in traditional and microspore-derived populations of Brassica juncea L. Czern. and Coss. Plant Breed 111:330–334
Upadhyay A, Mohapatra T, Pai RA, Sharma RP (1996) Molecular mapping and character tagging in Brassica juncea. II. Association of RFLP markers with seed coat colour and quantitative traits. J Plant Biochem Biotechnol 5:17–22
Vanghesdale G, Fournier N (1980) Composition chimique de la Brassica juncea utilisée dans la fabrication de la moutarde de Dijon. Rev Fr Corps Gras 1:15–22
Vaughan JG, Hemingway JS, Schofield HJ (1963) Contributions to a study of variation in Brassica juncea Czern. and Coss. J Linn Soc (Bot) 58:425–447
Vera CL, Woods DL (1982) Isolation of independent gene pairs at two loci for seed coat color in Brassica juncea. Can J Plant Sci 62:47–50
Vera CL, Woods DL, Downey RK (1979) Inheritance of seed coat color in Brassica juncea. Can J Plant Sci 59:635–637
Woods DL (1980) The association of yellow seed coat with other characters in mustard B. juncea. Cruciferae Newsl 5:23–24
Zeng Z (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by H.C. Becker
Rights and permissions
About this article
Cite this article
Lionneton, E., Aubert, G., Ochatt, S. et al. Genetic analysis of agronomic and quality traits in mustard (Brassica juncea). Theor Appl Genet 109, 792–799 (2004). https://doi.org/10.1007/s00122-004-1682-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-004-1682-0