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Fermentative production of succinic acid from glucose and corn steep liquor byAnaerobiospirillum succiniciproducens

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Abstract

Anaerobiospirillum succiniciproducens requires expensive complex nitrogen sources such as yeast extract and polypeptone for its growth and succinic acid production. It was found thatA. succiniciproducens was able to grow in a minimal medium containing glucose when supplemented with corn steep liquor (CSL) as the sole complex nitrogen source. The concentration of CSL had a significant effect on the glucose consumption byA. succiniciproducens. When 10–15 g/L of CSL was supplemented, cells were grown to an OD660 of 3.5 and produced 17.8 g/L succinic acid with 20 g/L glucose. These results are similar to those obtained by supplementing yeast extract and polypeptone, thereby suggesting that succinic acid can be produced more economically using glucose and CSL.

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Authors and Affiliations

  1. Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, 305-701, Taejon, Korea

    Pyung Cheon Lee, Woo Gi Lee, Sang Yup Lee, Ho Nam Chang & Yong Keun Chang

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  1. Pyung Cheon Lee
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  2. Woo Gi Lee
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  3. Sang Yup Lee
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  4. Ho Nam Chang
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  5. Yong Keun Chang
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Correspondence to Sang Yup Lee.

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Lee, P.C., Lee, W.G., Lee, S.Y. et al. Fermentative production of succinic acid from glucose and corn steep liquor byAnaerobiospirillum succiniciproducens . Biotechnol. Bioprocess Eng. 5, 379–381 (2000). https://doi.org/10.1007/BF02942216

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  • DOI: https://doi.org/10.1007/BF02942216

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