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Cloning and characterization ofMannheimia succiniciproducens MBEL55E phosphoenolpyruvate carboxykinase (pckA) gene

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Abstract

ApckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacteriumMannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of theM. succiniciproducens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKs.

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References

  1. Matte, A., L. W. Tari, H., Goldie, and L. T. J. Delbaere (1997) Structure and mechanism of phosphoenolpyruvate carboxykinase.J. Biol. Chem. 272: 8105–8108.

    Article  CAS  Google Scholar 

  2. Podkovyrove, S. M. and J. G. Zeikus (1993) Purification and characterization of phosphoenolpyruvate carboxykinase, a catabolic CO2-fixing enzyme, fromAnaerobiospirillum succiniciproducens.J. Gen. Microbiol. 139: 223–228.

    Google Scholar 

  3. Cannata, J. B. and M. A. C. de Flombaum (1974) Phosphoenolpyruvate carboxykinase from bakers yeast. Kinetics of phosphoenolpyruvate formation.J. Biol. Chem. 249: 3356–3365.

    CAS  Google Scholar 

  4. Colombo, G., G. M. Carlson, and H. A. Lardy (1981) Phosphoenolpyruvate carboxykinase (guanosine 5′-triphosphate) from rat liver cytosol. Dual-action requirement for the carboxylation reaction.Biochemistry 20: 2749–2757.

    Article  CAS  Google Scholar 

  5. Haarasilta, S. and E. Oura (1975) On the activity and regulation of anaplerotic and gluconeogenic enzymes during the growth process of baker's yeast.Eur. J. Biochem. 52: 1–7.

    Article  CAS  Google Scholar 

  6. Hansen, E. J. and E. Juni (1974) Two routes for synthesis of phosphoenolpyruvate from C4-dicarboxylic acids.Biochem. Biophys. Res. Commun. 59: 1204–1210.

    Article  CAS  Google Scholar 

  7. Kang, K. H. and H. W. Ryu (1999) Enhancement of succinate production by organic solvents, detergents, and vegetable oils.J. Microbiol. Biotechnol. 9: 191–195.

    CAS  Google Scholar 

  8. Hong, S. H. and S. Y. Lee (2000) Metabolic flux distribution in a metabolically engineered Escherichia coli strain producing succinic acid.J. Microbiol. Biotechnol. 10: 496–501.

    CAS  Google Scholar 

  9. Lee, P. C., W. G. Lee, S. Kwon, S. Y. Lee, and H. N. Chang (1999) Succinic acid production byAnaerobiospirillum succiniciproducens: Effects of the H2/CO2 supply and glucose concentration.Enzyme Microb. Technol. 24: 549–554.

    Article  CAS  Google Scholar 

  10. Lee, P. C., W. G. Lee, S. Y. Lee, and H. N. Chang (2000) Succinic acid production with reduced by-product formation the fermentation ofAnacrobiospirillum succiniciproducens using glycerol as a carbon source.Biotechnol. Bioeng. 72: 41–48.

    Article  Google Scholar 

  11. van der Werf, M. J., M. V. Guettler, M. K. Jain, and J. G. Zeikus (1997) Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation byActinobacillus sp. 120Z.Arch. Microbiol. 167: 332–342.

    Article  Google Scholar 

  12. Macy, J. M., L. G. Ljungdahl, and G. Gottschalk (1978) Pathway of succinate and propionate formation inBacteroides fragilis.J. Bacteriol. 134: 84–91.

    CAS  Google Scholar 

  13. Medina, V., R. Pontarollo, D. Glaeske, H. Tabel, and H. Goldie (1990) Sequence of thepckA gene ofEscherichia coli K-12: relevance to genetic and allosteric regulation and homology ofE. coli phosphoenolpyruvate carboxykinase with the enzymes fromTrypanosoma brucei andSaccharomyces cerevisiae.J. Bacteriol. 172: 7151–7166.

    CAS  Google Scholar 

  14. Østerås, M., B. T. Driscoll, and T. M. Finan (1995) Molecular and expression analysis of theRhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.J. Bacteriol. 177: 1452–1460.

    Google Scholar 

  15. Østerås, M., T. M. Finan, and J. Stanley (1991) Sitedirected mutagenesis and DNA sequence ofpckA ofRhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype.Mol Gen. Genet. 230: 257–269.

    Article  Google Scholar 

  16. Scovill, W. H., H. J. Schreier, and K. W. Bayles (1996) Identification and characterization of thepckA gene fromStaphylococcus aureus.J. Bacteriol. 178: 3362–3364.

    CAS  Google Scholar 

  17. Inui, M., K. Nakata, J. H. Roh, K. Zahn, and H. Yukawa (1999) Molecular and functional characterization of theRhodopseudomonas palustris no. 7 phosphoenolpyruvate carboxykinase gene.J. Bacteriol. 181: 2689–2696.

    CAS  Google Scholar 

  18. Laivenieks, M., C. Vieille, and J. G. Zeikus (1997) Cloning, sequencing, and overexpression of theAnaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene.Appl. Environ. Microbiol. 63: 2273–2280.

    CAS  Google Scholar 

  19. Lee, P. C., W. G. Lee, S. Y. Lee, H. N. Chang, and Y. K. Chang (2000) Fermentative production of succinic acid from glucose and corn steep liquor byAnaerobiospirillium succiniciproducens.Biotechnol. Bioprocess Eng. 5: 379–381.

    Article  CAS  Google Scholar 

  20. Sambrook, J., E. F. Fritsch, and T. Maniatis (1989)Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory press, Cold Spring Harbor, NY, USA.

    Google Scholar 

  21. Beckwith, J., J. Davies, and J. A. Gallant (1983)Gene Function in Prokarvotes. pp. 123–161. Cold Spring Harbor Laboratory press. Cold Spring Harbor, NY, USA.

    Google Scholar 

  22. Matte, A., H. Goldie, R. M. Sweet, and L. T. Delbaere (1996) Crystal structure ofEscherichia coli phosphoenolpyruvate carboxykinase: a new structural family with the P-loop nucleotiside triphosphate hydrolase fold.J. Mol. Biol. 256: 126–143.

    Article  CAS  Google Scholar 

  23. Tari, L. W., A. Matte, U. Pugazhenthi, H. Goldie, and L. T. Delbaere (1996) Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex ofEscherichia coli PEP carboxykinase.Nat. Struct. Biol. 3: 355–363.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea

    Pyung Cheon Lee, Sang Yup Lee & Soon Ho Hong

  2. Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea

    Pyung Cheon Lee, Sang Yup Lee & Ho Nam Chang

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  1. Pyung Cheon Lee
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  2. Sang Yup Lee
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  3. Soon Ho Hong
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  4. Ho Nam Chang
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Correspondence to Sang Yup Lee.

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Lee, P.C., Lee, S.Y., Hong, S.H. et al. Cloning and characterization ofMannheimia succiniciproducens MBEL55E phosphoenolpyruvate carboxykinase (pckA) gene. Biotechnol. Bioprocess Eng. 7, 95–99 (2002). https://doi.org/10.1007/BF02935886

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  • DOI: https://doi.org/10.1007/BF02935886

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