Summary
Hepatitis B surface antigen (HBs-Ag) and hepatitis B core antigen (HBc-Ag) in hepatic tissue of3 cases with various liver diseases were investigated by immunofluorescent method. Virus-like particles were demonstrated by electron microscopy in the nuclei of these 3 cases. The localization of HBs-Ag was restricted in cytoplasma or on the surface of hepatocyte, while HBc-Ag was almost in the hepatocytic nuclei. However, there was unexplainable discrepancy between the distribution of HBs-Ag and that of HBc-Ag, as more the former in number and less the latter, of hepatocytes. The size of virus-like particles in nuclei was 22–27 nm in diameter. Most of them were hollow, but some of them were wholly electron dense. Their distribution was various from case to case. Discussion was made on the correlation between the presence of Hepatitis B virus and HBs-Ag or HBc-Ag.
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References
Millman, I., Zavatone, V., Gerstley, B.J.S. and Blumberg, B.S.: Australia antigen detected in the nuclei of liver cells of patients with viral hepatitis by the fluorescent antibody technique. Nature 222: 181–184, 1969.
Nowoslawski, A., Brzosko, W.J., Madalinski, K. and Krawczynski, K.: Cellular localization of Australia antigen in the liver of patients with lymphoproliferative disorders. Lancet 1: 494–497, 1970.
Nielsen, J.O. and Elling, P.: Investigations of liver biopsies for Australia antigen by immunofluorescent Clin. Exp. Immunol. 9: 699–705, 1971.
Edgington, T.S. and Ritt, D.J.: Intrahepatic expression of serum hepatitis virus associated antigen. J. Exp. Med. 134: 871–885, 1971.
Hadziyannis, S., Moussouros, A., Vissoulis, C. and Afroudakis, A.: Cytoplasmic localization of Australia antigen in the liver. Lancet 1: 976–979, 1972.
Furuta, S., Hanaoka, S., Omori, A., Chiba, K., Tsukioka, J., Nagata, A., Takahashi, T., Kiyosawa, K., Akahane, Y. and Oda, M.: Australia antigen in serum and liver tissue of patients with various liver diseases. Kanzo 13: 280–287, 1972.
Müller, R., Maess, J. und Deicher, H.: Fluoreszenzserologischer Nachweis von Australia-antigen in der Leber. Dtsch. Med. Wschr. 98: 93–99, 1973.
Almeida, J.D., Bubenstein, D. and Scott, E.J.: New antigen-antibody system in Australia-antigenpositive hepatitis. Lancet 11: 1224–1227, 1971.
Hadziyannis, S., Moussouros, A., Giustozi, A. and Almeida, J.: Immunofluorescence study of the “core” antigen in the liver. Digestion 8: 437, 1973.
Shikata, T., Yoshizaki, C., Matsushita, H., Toyokawa, H. and Ishida, N.: Studies on the duality of intrahepatocytic HB-Ag by immunofluorescent antibody technique. Kanzo 15: 695, 1974.
Nelson, J.M., Barker, L.F. and Danovitch, S.H.: Intranuclear aggregates in the liver of a patient with serum hepatitis. Lancet 11: 773–774, 1970.
Huang, S.: Hepatitis-associated antigen hepatitis: Anectron microscopic study of virus-like particles in liver cells. Amer. J. Pathol. 64: 483–500, 1971.
Dunn, A.E.G., Peter, R.L., Schweitzer, I.L. and Spears, R.L.: Virus-like particles in livers of infants with vertically transmitted hepatitis. Arch. Pathol. 94: 258–264, 1972.
Scotto, J., Homberg, J.C. and Caroli, J.: Electron microscopy studies of severe virus hepatitis. Cytoplasmic inclusions, virus-like particles, Australia antigen. Amer. J. Dis. Child. 123: 311–312, 1972.
Campion, E.C., Ludbrook, J., McLeod, J.M., Marshall, V.R., Mukherjee, T. and Wangel, A.G.: Primary hepatoma and hepatitis-associated antigen in a young white woman. Brit. Med. J. 4: 149–150, 1972.
Caramia, F., DeBac, C. and Ricci, G.: Virus-like particles within hepatocytes of Australia antigen carriers. Amer. J. Dis. Child. 123: 309–311, 1972.
Hirschman, S.Z., Gerber, M. and Garfinkel, E.: Purification of naked intranuclear particles from human liver infected by hepatitis B virus. Proc. Nat. Acad. Sei. USA. 71: 3345–3349, 1974.
Tapp, E., Jones, D.M., Anfield, C., Whittaker, J.S., Woolf, I.L. and Dymock, J.W.: Intranuclear particles in hepatocytes of HB-Ag positive blood-donors. Lancet 1: 934, 1974.
Yamada, G., Kobayashi, T., Ohta, Y. and Kosaka, K.: HB-Ag associated particles in the sera and the liver of asymptomatic carriers. J. Clin. Electron Microscopy. 7: 368–369, 1974.
Kamimura, T., Murayama, H., Sasaki, H. and Ichida, F.: Electron microscopic studies on hepatocytes of asymptomatic carriers of hepatitis B antigen. ibid. 7: 370–371, 1974.
Barker, L.F., Almeida, J.D., Hoofnagle, J.H., Gerety, R.T., Jackson, D.R. and McGrath, P.P.: Hepatitis B core antigen: Immunology and electron microscopy. J. Virology. 14: 1552–1558, 1974.
Barker, L.F., Chisari, F.V., McGrath, P.P., Dalgard, D.W., Kirschstein, R.L., Almeida, J.D., Edgington, T.S., Sharp, D.G. and Peterson, M.R.: Transmission of type B viral hepatitis to chimpanzees. J. Infect. Dis. 127: 648–662, 1973.
Mayumi, M., Okochi, K. and Nishioka, K.: Detection of Australia antigen by means of immune adherence haemagglutination test. Vox Sang.: 20 178–181, 1971.
Hirschman, S.Z., Gerber, M. and Garfinkel, E.: DNA purified from naked intranuclear particles of human liver infected with hepatitis B virus. Nature. 251: 540–542, 1974.
Robinson, W.S. and Greenman, R.L.: DNA polymerase in the core of the human hepatitis B virus candidate. J. Virology. 13: 1231–1236, 1974.
Gerber, M.A., Hadziyannis, S., Vissoulis, C. Schffner, F., Paronetto, F. and Popper, H.: Hepatitis B antigen: Nature and distribution of cytoplasmic antigen in hepatocytes of carriers. Proc. Soc. Exp. Biol. Med. 145: 863–867, 1974.
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Furuta, S., Nagata, A., Kiyosawa, K. et al. HBs-Ag, HBc-Ag and virus-like particles in liver tissue. Gastroenterol Jpn 10, 208–214 (1975). https://doi.org/10.1007/BF02776654
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DOI: https://doi.org/10.1007/BF02776654