Summary
A comparison of the soluble and cell-wall-bound isoperoxidases of normal auxin-requiring and auxin-independent (habituated) tobacco callus revealed that normal tissues contained a higher level of isoperoxidases. There were also qualitative differences in these isoperoxidases. Partially purified soluble and ionically bound isoperoxidases of normal callus likewise exhibited higher auxin-oxidase activities. Normal tissues also were found to contain higher levels of auxin-oxidase inhibitors (auxin protectors). Overall, however, the data indicate that there is a higher rate of auxin destruction in normal tobacco callus than in habituated tissue. This presumably leads to insufficient endogenous auxin for growth.
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This study was supported in part by grants to T.G. from the Center IRSIA d'Etude de la Reproduction végétale and the FRFC Contract No. 2.9009. it Was carried out while T.A.T. was the holder of a senior Fellowship under the NATO senior Scientists Program.
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Bouchet, M., Gaspar, T. & Thorpe, T.A. Soluble and cell-wall peroxidases and auxin destruction in normal and habituated tobacco callus. In Vitro 14, 819–823 (1978). https://doi.org/10.1007/BF02616151
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DOI: https://doi.org/10.1007/BF02616151